Expression of minichromosome maintenance protein7 and cell division cycle protein6 and its significance in oral squamous cell carcinoma and precancerous lesion

doi:10.3877/cma.j.issn.1674-1366.2010.03.003

Abstract

Abstract:

Objective

To evaluate the expression of minichromosome maintenance protein7 （Mcm7） and cell division cycle protein 6 （Cdc6） and to determine its significance in oral squamous cell carcinoma （OSCC） and precancerous lesion.

Methods

RT-PCR and immunohistochemistry analysis were performed on 47 frozen samples and 98 paraffin-embedded samples to evaluate mRNA and protein expressions of Mcm7 and Cdc6.

Results

RT-PCR results indicated positive expressions of Mcm7 mRNA in normal oral mucosa， precancerous lesions， and squamous cell carcinomas and significant differences were found among different groups （P ＜0.01）.Cdc6 mRNA had low expression in normal oral mucosa and was highly expressed in precancerous lesions and squamous cell carcinoma， with significant differences delete among all groups （P ＜0.01）.Immunohistochemistry analysis results indicated positive protein expression of Mcm7 in the normal oral mucosa， precancerous lesions， and squamous cell carcinomas， with a labelling index of 3.6%， 22.3% and 45.9% respectively.Significant differences were found among all groups （P ＜0.01）.Mcm7 positive protein expression was significantly higher in the squamous cell carcinoma group than that of the precancerous lesion group （P ＜0.01）.Cdc6 positive protein expression was lower than Mcm7 expression in all samples， with low expressions observed in normal oral mucosa，and high expressions in precancerous lesions and squamous cell carcinomas.Labelling index values among groups were statistically significant （P ＜0.01）.Mcm7 and Cdc6 expressions in the lymph node metastasis group were significantly higher than those of the non-metastasis group （P ＜0.01）.

Conclusions

The expression of Mcm7 and Cdc6 is positively correlated to OSCC occurrence and lymph node metastasis.Detection of the expression of Mcm7 and Cdc6 may be used for early diagnosis and prognostic judgment of oral squamous cell carcinoma.

Key words: Oral squamous cell carcinoma, Precancerous lesion, Minichromosome maintenance protein7, Cell division cycle protein 6, RT-PCR, Immunohistochemistry

Hui-jun LI, Xiao-wei LU, Chun-yang LI, Ying-rong LAI, Chong-jin FENG, Gui-qing LIAO. Expression of minichromosome maintenance protein7 and cell division cycle protein6 and its significance in oral squamous cell carcinoma and precancerous lesion[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2010, 4(03): 224-231.

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Figures/Tables 5

References 12

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基因	序列	相对分子质量	退火温度（℃）
Mcm7	F：5'-GAGCATCGGCTAATGATGGAG-3'	268 000	60
	R：5'-GTGACGATTCCACGCACAGTT-3'
Cdc6	F：5'-TGCTCTTGATCAGGCAGTTGA-3'	363 000	60
	R：5'-GGCCCGAATGTGTAAAGCACT-3'
β-actin	F：5'-CATTGGCAATGAGCGGTTC-3'	115 000	60
	R：5'-CCACGTCACACTTCATGATGG-3'

基因	序列	相对分子质量	退火温度（℃）
Mcm7	F：5'-GAGCATCGGCTAATGATGGAG-3'	268 000	60
	R：5'-GTGACGATTCCACGCACAGTT-3'
Cdc6	F：5'-TGCTCTTGATCAGGCAGTTGA-3'	363 000	60
	R：5'-GGCCCGAATGTGTAAAGCACT-3'
β-actin	F：5'-CATTGGCAATGAGCGGTTC-3'	115 000	60
	R：5'-CCACGTCACACTTCATGATGG-3'

参数	例数	Mcm7 LI	P	Cdc6 LI	P
性别
男	29	47.0 ± 23.1		31.6±12.8
女	25	44.6 ± 24.5	0.713	30.7±16.9	0.894
肿瘤大小（cm）
≤2	13	38.0 ± 23.0		19.3± 9.0
＞ 2	41	48.4 ± 23.5	0.166	34.9±14.8	0.044
淋巴结转移情况
未转移	25	30.6 ± 16.5	＜0.001	19.7± 8.5	＜0.001
已转移	29	59.1 ± 20.6		41.0±15.1
临床分期
Ⅰ	7	22.4 ± 14.0		7.0± 4.2
Ⅱ	18	34.1 ± 16.6	＜0.001	24.7±11.5	＜0.001
Ⅲ	18	55.2 ± 16.7		37.1±16.1
Ⅳ	11	65.6 ± 25.4		47.4±18.3
病理分期
高分化	30	41.7 ± 24.1		28.5±12.9
中分化	12	48.8 ± 19.8		36.4±19.0
低分化	12	53.7 ± 25.1	0.298	32.5±14.0	0.673

参数	例数	Mcm7 LI	P	Cdc6 LI	P
性别
男	29	47.0 ± 23.1		31.6±12.8
女	25	44.6 ± 24.5	0.713	30.7±16.9	0.894
肿瘤大小（cm）
≤2	13	38.0 ± 23.0		19.3± 9.0
＞ 2	41	48.4 ± 23.5	0.166	34.9±14.8	0.044
淋巴结转移情况
未转移	25	30.6 ± 16.5	＜0.001	19.7± 8.5	＜0.001
已转移	29	59.1 ± 20.6		41.0±15.1
临床分期
Ⅰ	7	22.4 ± 14.0		7.0± 4.2
Ⅱ	18	34.1 ± 16.6	＜0.001	24.7±11.5	＜0.001
Ⅲ	18	55.2 ± 16.7		37.1±16.1
Ⅳ	11	65.6 ± 25.4		47.4±18.3
病理分期
高分化	30	41.7 ± 24.1		28.5±12.9
中分化	12	48.8 ± 19.8		36.4±19.0
低分化	12	53.7 ± 25.1	0.298	32.5±14.0	0.673

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