Bioinformatics analysis of differentially expressed mRNA in outer membrane vesicles of Porphyromonas gingivalis

doi:10.3877/cma.j.issn.1674-1366.2021.03.002

Abstract

Abstract:

Objective

To study the difference of mRNA expression profiles in the outer-membrane vesicles of Porphyromonas gingivalis W83 and ATCC33277.

Methods

The outer membrane vesicles derived from W83 and ATCC33277 were separated by ultracentrifugation. After the particle size detection, transmission electron microscopy, and Western blot, the outer membrane vesicles identified; the high-throughput sequencing technology was used to detect the mRNA expression profiles of outer membrane vesicles of W83 and ATCC33277. Membrane vesicle mRNA expression profile, which was screened out with differentially expressed mRNA, was performed by Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.

Results

Outer membrane vesicles isolated from W83 and ATCC33277 met the characteristics of vesicles. We identified 1629 mRNAs in W83 outer membrane vesicles, and 1505 mRNAs in ATCC33277 outer membrane vesicles and screened out differentially expressed mRNAs. A total of 594 items was identified. Through GO analysis and KEGG analysis of differentially expressed mRNAs, the pathways related to differential genes include biofilm Formation-Vibrio cholerae (P = 0.011) , ribosome (P = 0.015) , bacterial two-component regulation system (P = 0.026) with statistical significance (P<0.05) .

Conclusion

There are different expressed mRNA between W83 and ATCC33277.

Key words: Porphyromonas gingivalis, Outer membrane vesicles, High-Throughput screening assays, Two-component signal transduction system

Yi Ruan, Jun Ai, Chun Jiang, Luodan Zhao, Xin Xia, Mo Liu. Bioinformatics analysis of differentially expressed mRNA in outer membrane vesicles of Porphyromonas gingivalis[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2021, 15(03): 135-141.

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Figures/Tables 9

References 24

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组别	样本量	平均数	众数	标准差
ATCC33277	1 mL	128.1 ± 6.6	85.0 ± 6.2	53.6 ± 5.5
W83	1 mL	381.0 ± 3.9	381.9 ± 29.2	97.5 ± 2.0

组别	样本量	平均数	众数	标准差
ATCC33277	1 mL	128.1 ± 6.6	85.0 ± 6.2	53.6 ± 5.5
W83	1 mL	381.0 ± 3.9	381.9 ± 29.2	97.5 ± 2.0

基因	ATCC33277	W83	P值	描述
hagA	17	69	<0.000 000 36	血凝素hagA
mfa1	218	49	<0.000 000 71	Mfa1膜蛋白
mfa2	34	4	<0.000 829 45	Mfa1菌毛相关蛋白

基因	ATCC33277	W83	P值	描述
hagA	17	69	<0.000 000 36	血凝素hagA
mfa1	218	49	<0.000 000 71	Mfa1膜蛋白
mfa2	34	4	<0.000 829 45	Mfa1菌毛相关蛋白

通路名称	具有途径注释的候选基因	与途径相关的所有基因	P值
生物膜形成-霍乱弧菌	5	6	0.011
核糖体	24	54	0.015
细菌双组分调节系统	11	21	0.026

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