The effects of TAK-242 on the expression of TNF-α and Arg1 in diabetic mouse

doi:10.3877/cma.j.issn.1674-1366.2017.03.003

Abstract

Abstract:

Objective

To investigate the effect of TLR4 inhibitor TAK-242 on the expression of TNF-α in serum and TNF-α and Arg1 mRNA expression in periodontal tissues of diabetic mice and periodontitis mice.

Methods

Groups of diabetes, periodontitis and periodontitis combined with diabetes mouse models were successfully established and TAK-242 was applied in different groups separately. After 10 days, Elisa method was used to detect the expression of TNF-α in mouse serum and real time PCR was used to detect the mRNA expression of TNF-α and Arg1 in gingival tissue. One-Way ANOVA was applied to analyze the data.

Results

The expression of TNF-α in mice serum and gingival tissue was increased within diabetic group, periodontitis group and diabetic periodontitis group. The application of TAK-242 could reduce the expression level of TNF-α (F_serum= 25.329, P_serum<0.001; F_{gingival tissue}= 47.901, P_{gingival tissue}<0.001) . The expression of Arg1 in diabetes mellitus, periodontitis group and diabetic periodontitis group decreased in gingival tissues while upregulated after treated with TAK-242 (F= 45.897, P<0.001) .

Conclusions

TAK-242 could regulate the expression of TNF-α and Arg1 and might play an important role in the chronic inflammation of periodontitis, diabetes and diabetic periodontitis.

Key words: Diabetes mellitus, Periodontitis, Inflammation, chronic

Qianqian Han, Weijuan Qi, Zhao Liu, Xi Yang. The effects of TAK-242 on the expression of TNF-α and Arg1 in diabetic mouse[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2017, 11(03): 142-148.

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References 29

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基因	引物序列
GAPDH	上游5′-TGTGGGCATCAATGGATTTGG-3′
?	下游5′-ACACCATGTATTCCGGGTCAAT-3′
TNF-α	上游5′-CAGGCGGTGCCTATGTCTC-3′
?	下游5′-CGATCACCCCGAAGTTCAGTAG-3′
Arg1	上游5′-TGTCCCTAATGACAGCTCCTT-3′
?	下游5′-GCATCCACCCAAATGACACAT-3′

基因	引物序列
GAPDH	上游5′-TGTGGGCATCAATGGATTTGG-3′
?	下游5′-ACACCATGTATTCCGGGTCAAT-3′
TNF-α	上游5′-CAGGCGGTGCCTATGTCTC-3′
?	下游5′-CGATCACCCCGAAGTTCAGTAG-3′
Arg1	上游5′-TGTCCCTAATGACAGCTCCTT-3′
?	下游5′-GCATCCACCCAAATGACACAT-3′

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