To investigate the effect of IL-1β on cell proliferation, intracellular reactive oxygen species(ROS)level, and the invasion and migration ability of oral squamous cell carcinoma CAL-27 cell line and the non-small cell lung cancer A549 cell line.

Oral squamous cell carcinoma CAL27 cell line and non-small cell lung cancer A549 cell line, were treated with 0 ng/ml, 50 ng/ml and 100 ng/ml recombinant human IL-1β. The proliferation ability of CAL-27 and A549 cells were detected using CCK8 assay, the intracellular reactive oxygen species(ROS)levels were measured with Reactive Oxygen Species Assay Kit, the invasion and migration ability were investigated using Transwell assay. Statistical analysis of the results was performed using one-way ANOVA.

The proliferation ability of CAL-27 and A549 cells did not change significantly under treatment with IL-1β. The intracellular ROS levels of CAL-27 cells were up-regulated under treatment with IL-1β (50 and 100 ng/ml)for 8 and 12 h(F_{8 h}=24.436, P_{8 h}=0.001; F_{12 h}=64.579, P_{12 h} < 0.05), the intracellular ROS levels of A549 cells were upregulated under treatment with IL-1β(50 and 100 ng/ml)for 4, 8 and 12 h(F_{4 h}=19.888, P_{4 h}=0.002; F_{8 h}=30.249, P_{8 h} < 0.05; F_{12 h}=32.703, P_{12 h}=0.001). The invasion and migration ability of CAL-27 and A549 cells were obviously increased by recombinant human IL-1β(F_{CAL-27 invasion}=159.783, P_{CAL-27 invasion} < 0.05; F_{CAL-27 migration}=264.045, P_{CAL-27 migration} < 0.05; F_{A549 invasion}=936.278, P_{A549 invasion} < 0.05; F_{A549 migration}=1389.961, P_{A549 migration} < 0.05).

IL-1β could increase the ROS level as well as the invasion and migration ability of oral squamous cell carcinoma CAL-27 cells and the non-small cell lung cancer A549 cells.