Expression of focal adhesion kinase and vascular endothelial growth factor in salivary adenoid cystic carcinoma and their significances

doi:10.3877/cma.j.issn.1674-1366.2015.04.003

Abstract

Abstract:

Objective

To investigate the expression of focal adhesion kinase(FAK)and vascular endothelial growth factor(VEGF)in salivary adenoid cystic carcinoma(SACC)and their significances.

Methods

The expressions of FAK, VEGF and CD34 in 40 cases of SACC and 15 cases of normal salivary gland tissue were detected with immunohistochemical method. SPSS 19.0 software was used for Mann-Whitney U, Chi-square test, Fisher′s exact test and correlation analysis.

Results

The expression of FAK was seen in 87.5% of SACC and 33.33% in the control group, Z=-4.44, P=0.001; The expression of VEGF was 90% and 26.67% respectively, Z=-4.84, P=0.001; The expression of CD34 was 85% and 40% respectively, Z=-4.18, P=0.002. The differences were significant(P < 0.05). The expression of FAK and VEGF in SACC was associated with tumor angiogenesis(F_FAK=8.86、P_FAK=0.01; F_VEGF=8.18、P_VEGF=0.01). The expression of FAK and VEGF was significantly associated with clinical stage and metastasis in the samples(P < 0.05), but did not correlate with sex, age, tumor diameter and pathological grade(P > 0.05). The expression of FAK was positively correlated with the expression of VEGF. The coefficient of correlation was 0.479, P=0.002.

Conclusion

The expression of FAK and VEGF is significantly associated with tumor clinical stage clinical stage and metastasis.

Key words: Salivary adenoid cystic carcinoma, Metastasis, Focal adhesion kinase, Vascular endothelial growth factor, Microvessel density

Pan Gong, Kexiong Ou-Yang, Kai Chen, Yanhao Kuang, Jinsong Li. Expression of focal adhesion kinase and vascular endothelial growth factor in salivary adenoid cystic carcinoma and their significances[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(04): 272-276.

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https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2015/V09/I04/272

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References 12

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表达强度	FAK		VEGF
表达强度	例数	MVD(±s)	例数	MVD(±s)
低表达	9	70.61 ± 7.79	8	73.05 ± 8.62
高表达	31	95.77 ± 8.72	32	91.16 ± 9.57

表达强度	FAK		VEGF
表达强度	例数	MVD(±s)	例数	MVD(±s)
低表达	9	70.61 ± 7.79	8	73.05 ± 8.62
高表达	31	95.77 ± 8.72	32	91.16 ± 9.57

临床病理		FAK		P值	VEGF		P值
临床病理		低表达	高表达	P值	低表达	高表达	P值
性别		?	?	0.26	?	?	0.13
?	男	4(16.00)	21(84.00)	?	3(12.00)	22(88.00)	?
?	女	5(33.33)	10(64.67)	?	5(33.33)	10(66.67)	?
年龄(岁)		?	?	0.26	?	?	0.12
?	< 50	6(31.58)	13(68.42)	?	6(31.58)	13(68.42)	?
?	≥50	3(14.29)	18(85.71)	?	2(9.52)	19(90.48)	?
发生部位		?	?	0.12	?	?	0.40
?	大唾液腺	4(14.81)	23(85.19)	?	4(14.81)	23(85.19)	?
?	小唾液腺	5(38.46)	8(61.54)	?	4(30.77)	9(69.23)	?
T分类		?	?	0.17	?	?	0.35
?	T₁+T₂	4(44.44)	5(55.56)	?	3(33.33)	6(66.67)	?
?	T₃+T₄	5(16.13)	26(83.87)	?	5(16.13)	26(83.87)	?
淋巴结转移		?	?	0.31	?	?	0.26
?	有	2(40.00)	3(60.00)	?	2(40.00)	3(60.00)	?
?	无	7(20.00)	28(80.00)	?	6(17.14)	29(82.86)	?
远处转移		?	?	0.02	?	?	0.02
?	有	0(0.00)	15(100.00)	?	0(0.00)	15(100.00)	?
?	无	9(36.00)	16(64.00)	?	8(32.00)	17(68.00)	?
TNM分期		?	?	0.01	?	?	0.04
?	Ⅰ+Ⅱ期	6(50.00)	6(50.00)	?	5(41.67)	7(58.33)	?
?	Ⅲ+Ⅳ期	3(10.71)	25(89.29)	?	3(10.71)	25(89.29)	?
病理分级		?	?	0.43	?	?	0.31
?	Ⅰ级	2(12.50)	14(87.50)	?	2(12.50)	14(87.50)	?
?	Ⅱ级	4(26.67)	11(73.33)	?	4(26.67)	11(73.33)	?
?	Ⅲ级	3(33.33)	6(66.67)	?	2(22.22)	7(77.78)	?
复发		?	?	0.13	?	?	0.19
?	有	4(40.00)	6(60.00)	?	4(40.00)	6(60.00)	?
?	无	5(16.67)	25(83.33)	?	4(13.33)	26(86.67)	?
神经侵犯		?	?	0.41	?	?	0.21
?	有	4(33.33)	8(66.67)	?	4(33.33)	8(66.67)	?
?	无	5(17.86)	23(82.14)	?	4(14.29)	24(85.71)	?

临床病理		FAK		P值	VEGF		P值
临床病理		低表达	高表达	P值	低表达	高表达	P值
性别		?	?	0.26	?	?	0.13
?	男	4(16.00)	21(84.00)	?	3(12.00)	22(88.00)	?
?	女	5(33.33)	10(64.67)	?	5(33.33)	10(66.67)	?
年龄(岁)		?	?	0.26	?	?	0.12
?	< 50	6(31.58)	13(68.42)	?	6(31.58)	13(68.42)	?
?	≥50	3(14.29)	18(85.71)	?	2(9.52)	19(90.48)	?
发生部位		?	?	0.12	?	?	0.40
?	大唾液腺	4(14.81)	23(85.19)	?	4(14.81)	23(85.19)	?
?	小唾液腺	5(38.46)	8(61.54)	?	4(30.77)	9(69.23)	?
T分类		?	?	0.17	?	?	0.35
?	T₁+T₂	4(44.44)	5(55.56)	?	3(33.33)	6(66.67)	?
?	T₃+T₄	5(16.13)	26(83.87)	?	5(16.13)	26(83.87)	?
淋巴结转移		?	?	0.31	?	?	0.26
?	有	2(40.00)	3(60.00)	?	2(40.00)	3(60.00)	?
?	无	7(20.00)	28(80.00)	?	6(17.14)	29(82.86)	?
远处转移		?	?	0.02	?	?	0.02
?	有	0(0.00)	15(100.00)	?	0(0.00)	15(100.00)	?
?	无	9(36.00)	16(64.00)	?	8(32.00)	17(68.00)	?
TNM分期		?	?	0.01	?	?	0.04
?	Ⅰ+Ⅱ期	6(50.00)	6(50.00)	?	5(41.67)	7(58.33)	?
?	Ⅲ+Ⅳ期	3(10.71)	25(89.29)	?	3(10.71)	25(89.29)	?
病理分级		?	?	0.43	?	?	0.31
?	Ⅰ级	2(12.50)	14(87.50)	?	2(12.50)	14(87.50)	?
?	Ⅱ级	4(26.67)	11(73.33)	?	4(26.67)	11(73.33)	?
?	Ⅲ级	3(33.33)	6(66.67)	?	2(22.22)	7(77.78)	?
复发		?	?	0.13	?	?	0.19
?	有	4(40.00)	6(60.00)	?	4(40.00)	6(60.00)	?
?	无	5(16.67)	25(83.33)	?	4(13.33)	26(86.67)	?
神经侵犯		?	?	0.41	?	?	0.21
?	有	4(33.33)	8(66.67)	?	4(33.33)	8(66.67)	?
?	无	5(17.86)	23(82.14)	?	4(14.29)	24(85.71)	?

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