The effect of hRpn13 （a subunit of 26S proteasome） regulating human periodontal ligament cells

doi:10.3877/cma.j.issn.1674-1366.2014.02.004

Abstract

Abstract:

Objective

To investigate the role of human regulatory particle non-ATPase 13（hRpn13） on the ubiquitin-26S proteasome pathway in human periodontal ligament cells （PDLCs） and the effects of hRpn13 on cell proliferation， differentiation， and function of human PDLCs.

Methods

The effects of altered hRpn13 gene expression on cell proliferation， apoptosis and differentiation of PDLCs were examined by MTT assay， DAPI staining， ALP ELISA Kit and elevation of collagenⅠ. And κappa B ligand/osteoprotegerin （RANKL/OPG） and ubiquitin gene expression was detectd by western blot.

Results

hRpn13 played a negative role in the proliferation， ALP activity and expression of collagen Ⅰ， RANKL and OPG. Moreover， the expression of ubiquitined protein decreased with over expression of hRpn13.

Conclusion

HRpn13 can influence cell proliferation， differentiation， and function of human PDLCs by the ubiquitin-26S proteasome pathway.

Key words: Human regulatory particle non-ATPase 13, Ubiquitin-26S proteasome pathway, Periodontal ligament cell, Osteoblast differentiatio

Xi Zhao, Yonglie Chao, Pixiu Chen, Liehua Qin. The effect of hRpn13 （a subunit of 26S proteasome） regulating human periodontal ligament cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(02): 104-111.

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Figures/Tables 6

References 26

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时间（h）	Scramble siRNA	hRpn13 siRNA	Empty vector	hRpn13 vector
48	0.744 ± 0.024	0.732 ± 0.032	0.709 ± 0.019	0.677 ± 0.034
72	0.827 ± 0.033	0.912 ± 0.021	0.794 ± 0.021	0.640 ± 0.024

时间（h）	Scramble siRNA	hRpn13 siRNA	Empty vector	hRpn13 vector
48	0.744 ± 0.024	0.732 ± 0.032	0.709 ± 0.019	0.677 ± 0.034
72	0.827 ± 0.033	0.912 ± 0.021	0.794 ± 0.021	0.640 ± 0.024

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