An in-situ remineralization design of hybrid layer with the use of sodium tripolyphosphate as a therapeutic primer

doi:10.3877/cma.j.issn.1674-1366.2014.02.003

Abstract

Abstract:

Objective

To test the hypothesis that the application of asodium tripolyphosphate（STTP） primer and a Portland cement-based lining composite on hybrid layers can induce intrafibrillar remineralization in the presence of the amorphous calcium phosphate （ACP）-stabilization analog supplied in simulated body fluid （SBF）.

Methods

Together with Portland cement-based composite， biomimetic remineralization system was built by phosphorylating collagen with 0.245 mol/L STTP for 5 min and by adding polyacrylic acid （PAA） to SBF. Based on these results， a in-situ mineralization scheme was developed for chemical phosphorylation of acid-etched dentin with 0.245 mol/L STTP， followed by infiltration of the STTP-treated collagen matrix with One-Step. Resin dentin interfaces were then capped with a Portland cement-based hydrophilic composite a liner， and remineralized in SBF with the use of PAA as an ACP-stabilization analog. Remineralized resin-dentin interfaces were examined unstained using transmission electron microscopy （TEM）.

Results

Periodic nanocomposite assembly with collagen sponge as a template was demonstrated with TEM using a Portland cement-based resin composite and a 5-minute STTP phosphorylation. Apatite was detected within the collagen at 72 h， and heavily deposited in collagen fibrils with periodically arranged intrafibrillar apatite platelets at 28 d. Infrared spectrum of the mineralized collagen sponge demonstrated characteristic hydroxyl apatite peaks at 900 ～1200 cm ^-1 and 500 ～600 cm^-1. The results of that model were further validated by complete remineralization of the hybrid layers treated with 0.245 mol/L STTP as a therapeutic primer and lined with a Portland cementbased lining composite， and with the ACP-stabilization analog supplied in SBF.

Conclusions

The STTP primer and the Portland cement-based lining composite employed in this study may be used with the ACP-stabilization analogy for in-situ remineralization of hybrid layer. This provides a potential delivery system for realization of the goal of biomimetic remineralization of dentin hybrid layer.

Key words: TypeⅠcollagen, Hybrid layers, In-situ remineralization, Sodium tripolyphosphate, Portland cement-based composite

Lisha Gu, Yipin Qi, Sui Mai, Qi Huang, Junqi Ling. An in-situ remineralization design of hybrid layer with the use of sodium tripolyphosphate as a therapeutic primer[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(02): 97-103.

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Figures/Tables 4

References 12

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