Osteogenesis of rat bone marrow stromal cells in vitro and in vivo

doi:10.3877/cma.j.issn.1674-1366.2011.02.003

Abstract

Abstract:

Objective

Observing the osteogenesis of rat bone marrow stromal cells（BMSCs） in osteogenic medium in vitro and in vivo.

Methods

BMSCs were harvested from rats and induced in control medium （CM） and osteogenic medium （OM）.Alkaline phosphatase （ALP）activity assay and von Kossa staining were used to assess the osteogenic differentiation.In vivo OM induced BMSCs were seeded with osteostimulative scaffold PerioGlas and implanted into subcutaneous nude mice to assess the ability of bone formation.

Results

BMSCs underwent osteogenic differentiation in osteogenic medium， showed higher level of ALP activity and matrix mineralization.Extensive neo-bone formation was observed after 8 weeks of implantation in PerioGlas with OM induced BMSCs.

Conclusions

BMSCs cultured in osteogenic medium commits to osteoblastic differentiation in vitro and induced ectopic bone formation with PerioGlas in vivo.

Key words: Bone marrow stromal cells, Osteogenesis, Differentiation, PerioGlas

Shi-hu SHENG, Qian-ting HE, Zhong-hua LIU, An-xun WANG. Osteogenesis of rat bone marrow stromal cells in vitro and in vivo[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(02): 126-131.

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Figures/Tables 4

References 17

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时间（d）	OM组	CM组
3	1.54±0.58^a	1.11±0.14^a
6	4.60±0.62^b	2.43±0.29^b
9	12.76±2.20^c	3.02±0.74^c

时间（d）	OM组	CM组
3	1.54±0.58^a	1.11±0.14^a
6	4.60±0.62^b	2.43±0.29^b
9	12.76±2.20^c	3.02±0.74^c

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