Apple polyphenols inhibiting ACC-M cell proliferation： results of in vitro study

doi:10.3877/cma.j.issn.1674-1366.2011.02.002

Abstract

Abstract:

Objective

To investigate the mechanism of apple polyphenols inhibiting ACCM cell proliferation in in vitro.

Methods

The effects of apple polyphenols on the proliferation in ACC-M cell was observed by 3-（4， 5-dimethylthiazol-2-yl）-2， 5-diphenyl tetrazolium bromide（MTT） assay and flow cytometry.Eeffects of apple polyphenols on the mRNA expression of VEGFR2 in ACC-M cell was evaluated by RT-PCR.Western blot was used to observed the influences of apple polyphenols on the protein of VEGFR2 in ACC-M cell.The data were analyzed by SPSS 13.0 software package.

Results

Compared with the control group， proliferation of ACCM cell in experimental group decreased and VEGFR2 mRNA and protein expression was down regulated significantly with the increase in the concentration of apple polyphenol.

Conclusions

Apple polyphenols could inhibit cell proliferation in ACC-M cell in vitro， and one of the mechanisms was probably to restrain the expression of the mRNA and protein of VEGFR2.

Key words: Apple polyphenols, ACC-M, VEGFR2

Chao-qun ZHENG, Hai-kuo TANG, Biao LV, Zhi-feng CHEN, Qian TAO. Apple polyphenols inhibiting ACC-M cell proliferation： results of in vitro study[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(02): 118-125.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2011.02.002

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Figures/Tables 11

References 16

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基因	引物序列	产物大小
VEGFR2	F：5'AGAAGATAAACCAGGCAAGG3'	349 000
	R：5'TCTCAAGGAAGGTCTGGCAA3'
β-actin	F：5'CCTTCCTGGGCATGGAGTCCT3'	205 000
	R：5'GGAGCAATGATCTTGATCAAC3'

基因	引物序列	产物大小
VEGFR2	F：5'AGAAGATAAACCAGGCAAGG3'	349 000
	R：5'TCTCAAGGAAGGTCTGGCAA3'
β-actin	F：5'CCTTCCTGGGCATGGAGTCCT3'	205 000
	R：5'GGAGCAATGATCTTGATCAAC3'

组别	剂量（μg/ml）	吸光度（A）值
组别	剂量（μg/ml）	24 h	48 h
对照组	0	0.765±0.008	1.756±0.05
实验组	50	0.771±0.020	1.760±0.03
	100	0.764±0.010	1.685±0.06^a
	150	0.768±0.020	1.475±0.03^b
	200	0.732±0.040^a	1.103±0.05^b
	250	0.566±0.020^b	0.893±0.06^b
	300	0.336±0.010^b	0.624±0.04^b
	500	0.304±0.010^b	0.458±0.05^b

组别	剂量（μg/ml）	吸光度（A）值
组别	剂量（μg/ml）	24 h	48 h
对照组	0	0.765±0.008	1.756±0.05
实验组	50	0.771±0.020	1.760±0.03
	100	0.764±0.010	1.685±0.06^a
	150	0.768±0.020	1.475±0.03^b
	200	0.732±0.040^a	1.103±0.05^b
	250	0.566±0.020^b	0.893±0.06^b
	300	0.336±0.010^b	0.624±0.04^b
	500	0.304±0.010^b	0.458±0.05^b

组别	剂量（μg/ml）	吸光度（A）值
组别	剂量（μg/ml）	24 h	48 h
对照组	0	0.931±0.07	1.886±0.04
实验组	50	0.942±0.03	1.892±0.01
	100	0.925±0.01	1.875±0.05
	150	0.955±0.04	1.895±0.02
	200	0.939±0.05	1.877±0.01
	250	0.904±0.04	1.873±0.03
	300	0.810±0.02^a	1.632±0.02^a
	500	0.727±0.05^a	1.515±0.03^a

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