The expression and modulatory effect of fibroblast growth factor 2 and bone morphogenetic protein 2 on the differentiation of dental derived cells

doi:10.3877/cma.j.issn.1674-1366.2011.01.002

Abstract

Abstract:

Objective

The purpose of this study was to investigate the expression and distribution of fibroblast growth factor 2 （FGF2） and bone morphogenetic protein 2 （BMP2） in human dental pulp cells （DPCs） and periodontal ligament cells （PDLCs） undergoing odontogenic/osteogenic differentiation and rat dental pulp and periodontal ligament with injury.

Methods

DPCs and PDLCs were isolated by enzymatic digestion and preceded to odontogenic/osteogenic induction for 14 d， and the cells without induction served as controls. The expressions of FGF2 and BMP2 were investigated by quantitative real-time RT-PCR. Lewis rat model with dental pulp and periodontal ligament injury was created and the distribution of FGF2 and BMP2 was determined by immunofluorescent staining.

Results

The mRNA level of BMP2 was significantly increased albeit FGF2 significantly decreased compared with the control group as DPCs and PDLCs undergoing odontogenic/osteogenic induction （P＜0.05）. Immunofluorescent staining also revealed the FGF2 merely showed tense expression in the regenerated peridontal ligament tissue，whereas BMP2 signal was activated both in the newly formed dental pulp and periodontal ligament tissue compared with the normal ones.

Conclusions

FGF2 and BMP2 signaling may play individual modulatory role in the odontogenic/osteogenic differentiation of dental derived cells with in vitro culture， albeit FGF2 and BMP2 may cooperatively regulate the in vivo dental tissue regeneration after injury.

Key words: Dental pulp cells, Periodontal ligament cells, Odontogenic/osteogenic differentiation, Tissue regeneration

Lu LIU, Xi WEI, Jun-qi LING, Li-ping WU. The expression and modulatory effect of fibroblast growth factor 2 and bone morphogenetic protein 2 on the differentiation of dental derived cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(01): 5-11.

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Figures/Tables 8

References 15

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基因	引物序列
FGF2	上游：5'-AGCCAGGTAACGGTTAGCAC-3'
	下游：5'-GGAGAAGAGCGACCCTCAC-3'
BMP2	上游：5'-CCTCCGTGGGGATAGAACTT-3'
	下游：5'-CACTGTGCGCAGCTTCC-3'
GAPDH	上游：5'-AGATCATCAGCAATGCCTCCTGC-3'
	下游：5'-ATGGCATGGACTGTGGTCATGAG-3'

基因	引物序列
FGF2	上游：5'-AGCCAGGTAACGGTTAGCAC-3'
	下游：5'-GGAGAAGAGCGACCCTCAC-3'
BMP2	上游：5'-CCTCCGTGGGGATAGAACTT-3'
	下游：5'-CACTGTGCGCAGCTTCC-3'
GAPDH	上游：5'-AGATCATCAGCAATGCCTCCTGC-3'
	下游：5'-ATGGCATGGACTGTGGTCATGAG-3'

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