The effects of simulating static magnetic fields of magnetic attachments on the capability of mineralization of osteoblasts

doi:10.3877/cma.j.issn.1674-1366.2010.05.007

Abstract

Abstract:

Objective

To study the effects of the various static magnetic field （SMF）intensity of the magnetic attachment system on the alkaline phosphatase （ALP） activity and the mineralization capability of the osteoblast.

Methods

The in vitro cultured SD rat osteoblasts were exposed continuously to 12.5 mT， 125 mT， and 250 mT SMF for 1，3，5，and 7 days in the test groups， while the control group without SMF exposure were used for comparison.The activity of cell ALP was measured with a spectrophotometer and the capability of mineralization was measured by alizarin red S （ARS） staining.Statistical analysis was performed.

Results

For 1-d SMF exposure， there was no statistical significance （P ＞0.05） in the ALP activity of osteoblast among all the testing groups and the control groups.For 3-d SMF exposure， statistical significances （P ＜0.05） were found between the 125， 250 mT group and the control group.When the SMF exposure time was extended to 5 and 7 days， the ALP activities of all the testing groups showed significant differences from the control group （P ＜0.01）.In addition， the ALP activities of the 125 mT and 250 mT groups were higher than that of the 12.5 mT group.The number of the mineral nodes of the three exposed groups was significantly larger than that of the control group.

Conclusions

Static magnetic field of magnetic attachment can stimulate the ALP activity and the mineralization capability of the osteoblast under the conditions defined in this study.

Key words: Magnetic attachments, Static magnetic fields, Osteoblast, Alkaline phosphatase

Yu ZHAO, Bing-yan LI, Li DU, Yong-lie CHAO. The effects of simulating static magnetic fields of magnetic attachments on the capability of mineralization of osteoblasts[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2010, 4(05): 471-476.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2010.05.007

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2010/V4/I05/471

Figures/Tables 5

References 13

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组别	静磁场加载时间（d）				P
组别	1	3	5	7	P
对照组	3.239 ± 0.062	8.748 ± 0.049	10.512 ± 0.098	12.840 ± 0.126	0.030
12.5 mT 组	2.973 ± 0.102	8.143 ± 0.495	15.336 ± 2.272*	26.923 ± 1.608*	0.002
125 mT 组	3.003 ± 0.309	12.321 ± 0.691*	28.803 ± 1.072*^#	46.262 ± 3.211*^#	0.000
250 mT 组	2.753 ± 0.411	11.275 ± 1.091*	27.897 ± 1.460*^#	48.416 ± 1.570*^#	0.000
P	0.189	0.001	0.000	0.000	0.000^△

组别	静磁场加载时间（d）				P
组别	1	3	5	7	P
对照组	3.239 ± 0.062	8.748 ± 0.049	10.512 ± 0.098	12.840 ± 0.126	0.030
12.5 mT 组	2.973 ± 0.102	8.143 ± 0.495	15.336 ± 2.272*	26.923 ± 1.608*	0.002
125 mT 组	3.003 ± 0.309	12.321 ± 0.691*	28.803 ± 1.072*^#	46.262 ± 3.211*^#	0.000
250 mT 组	2.753 ± 0.411	11.275 ± 1.091*	27.897 ± 1.460*^#	48.416 ± 1.570*^#	0.000
P	0.189	0.001	0.000	0.000	0.000^△

组别	矿化结节数
对照组	23.1 ± 3.6
12.5 mT 组	32.6 ± 4.8*
125 mT 组	34.3 ± 3.9*
250 mT 组	30.5 ± 4.5*

组别	矿化结节数
对照组	23.1 ± 3.6
12.5 mT 组	32.6 ± 4.8*
125 mT 组	34.3 ± 3.9*
250 mT 组	30.5 ± 4.5*

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