Study of released metal ions of three kinds of dental casting alloy crowns and DNA damage of mucosal cells in vitro

doi:10.3877/cma.j.issn.1674-1366.2008-01-008

Abstract

Abstract:

Objective

To study the corrosion behavior of three kinds of dental casting alloys and investigate the DNA damage of dog buccal mucosal cells caused by the released metal ions.

Methods

Three kinds of commonly-used dental casting alloys （pure Ti， Co-Cr-Mo and Ni-Cr） were used to make full crowns for dogs. The type and concentration of the released metal ions were measured by ICP-AES 2 weeks， 1 month， 2 months and 3 months after restoration. The DNA damage of buccal mucosal cells was studied by SCGE. Data were statistically analyzed by analysis of variance （ANOVA） and Spearman correlation.

Results

The metal ions released from Co-Cr-Mo and Ni-Cr were detected in buccal mucosal cells while the amount of the ions released from the pure Ti was too small to be detected. The DNA damage of mucosal cells increased with time after restoration of Ni-Cr crowns.

Conclusions

Pure Ti was the most corrosion-resistant among the three alloys. Pure Ti and Co-Cr-Mo showed no cytotoxicity.Ni-Cr alloy was prone to corrode and has cytotoxicity.

Key words: Dental casting alloy, Ion releasing, Cells, DNA

Jian-sheng SU, Zhi-juan TIAN, Shan GUO, Lei SHAO. Study of released metal ions of three kinds of dental casting alloy crowns and DNA damage of mucosal cells in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(01): 36-40.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2008-01-008

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2008/V02/I01/36

Figures/Tables 2

References 12

1	Wataha JC. Alloys for prosthodontic restorations. J Prosthet Dent， 2002，87（4）：351-363.
2	Shettlemore MG， Bundy KJ. Assessment of dental material degradation product toxicity using a bioluminescent bacterial assay. Dent Mater， 2002，18（6）：445-453.
3	Trombetta D， Mondello MR， Cimino F， et al. Toxic effect of nickel in an in vitro model of human oral epithelium. Toxicolo Lett， 2005，159（3）：219-225.
4	何惠明，武红艳，张春宝，等. 不同牙科合金材料对龈沟液中某些细胞因子含量的影响. 中国临床康复， 2005，9（14）：48-49.
5	Faccioni F， Franceschetti P， Cerpelloni M， et al. In vivo study on metal release from fixed orthodontic appliances and DNA damage in oral mucosa cells. Am J Orthod Dentofacial Orthofacil Orthop， 2003，124（6）：687-694.
6	苏俭生，邓章悦，邵磊，等. 铸造合金全冠戴用后颊黏膜上皮细胞DNA 损伤的研究. 华西口腔医学杂志， 2006，24（1）：21-25.
7	刘肃，周延民，王晓容，等. 镍铬合金、钛合金及铂合金在人工唾液中的耐腐蚀性能. 吉林大学学报（医学版）， 2005， 31（5）：774-777.
8	Robert GC， John MP. 牙科修复材料学. 赵信义，易超，译. 西安：世界图书出版西安公司， 2006：315.
9	Matkovi T， Matkovi P， Malina J. Effects of Ni and Mo on the microstructure and some other properties of Co-Cr dental alloys. Journal of Alloys and Compounds， 2004， 366（1-2）：293-297.
10	Sinko I， Morocz M， Zadori J， et al. Effect of cigarette smoking on DNA damage of human cumulus cells analyzed by comet assay.Reprod Toxicol， 2005，20（1）：65-71.
11	Choucroun P， Gillet D， Dorange G， et al. Comet assay and early apoptosis. Mutat Res， 2001，478（1-2）：89-96.
12	Kido R， Sato I， Tsuda S. Detection of in vivo DNA damage induced by ethanol in multiple organs of pregnant mice using the alkaline single cell gel electrophoresis （Comet） assay. J Vet Med Sci， 2006，68（1）：41-47.

组别	金属离子	戴冠前	戴冠2周	戴冠1月	戴冠2月	戴冠3月	F（X）	P
纯钛组	Fe	1.6±0.8	1.7±0.5	1.9±0.6	1.9±0.4	2.6±0.6	1.99	0.188 7
	Ti	0.000 7±0.000 1	0.000 7±0.000 1	0.000 7±0.000 1	0.000 6±0.000 2	0.000 7±0.000 3	0.29	0.874 7
钴铬钼	Cr	0.52±0.11	0.73±0.08	0.81±0.08	0.95±0.10	1.05±0.05	30.66	0.000 1
合金组	Mo	0.45±0.21	0.43±0.18	0.43±0.21	0.51±0.09	0.51±0.11	0.14	0.964 6
	Co	0.20±0.10	0.40±0.08	0.52±0.07	0.59±0.08	0.60±0.04	117.45	0.000 1
镍铬合	Cr	0.58±0.06	0.98±0.06	1.49±0.04	2.05±0.09	2.02±0.20	135.21	0.000 1
金组	Mo	0.438±0.034	0.455±0.026	0.49±0.06	0.47±0.09	0.50±0.12	0.74	0.591 5
	Ni	0.75±0.10	1.98±0.12	1.80±0.14	2.63±0.14	2.66±0.06	396.03	0.000 1
	Be	0.154±0.016	0.501±0.037	0.88±0.09	1.46±0.10	1.51±0.20	147.38	0.000 1
	Al	5.6±1.0	6.1±1.0	5.8±0.8	6.3±0.8	5.5±0.9	0.47	0.775 6

组别	金属离子	戴冠前	戴冠2周	戴冠1月	戴冠2月	戴冠3月	F（X）	P
纯钛组	Fe	1.6±0.8	1.7±0.5	1.9±0.6	1.9±0.4	2.6±0.6	1.99	0.188 7
	Ti	0.000 7±0.000 1	0.000 7±0.000 1	0.000 7±0.000 1	0.000 6±0.000 2	0.000 7±0.000 3	0.29	0.874 7
钴铬钼	Cr	0.52±0.11	0.73±0.08	0.81±0.08	0.95±0.10	1.05±0.05	30.66	0.000 1
合金组	Mo	0.45±0.21	0.43±0.18	0.43±0.21	0.51±0.09	0.51±0.11	0.14	0.964 6
	Co	0.20±0.10	0.40±0.08	0.52±0.07	0.59±0.08	0.60±0.04	117.45	0.000 1
镍铬合	Cr	0.58±0.06	0.98±0.06	1.49±0.04	2.05±0.09	2.02±0.20	135.21	0.000 1
金组	Mo	0.438±0.034	0.455±0.026	0.49±0.06	0.47±0.09	0.50±0.12	0.74	0.591 5
	Ni	0.75±0.10	1.98±0.12	1.80±0.14	2.63±0.14	2.66±0.06	396.03	0.000 1
	Be	0.154±0.016	0.501±0.037	0.88±0.09	1.46±0.10	1.51±0.20	147.38	0.000 1
	Al	5.6±1.0	6.1±1.0	5.8±0.8	6.3±0.8	5.5±0.9	0.47	0.775 6

组别	戴冠前	戴冠2周	戴冠1个月	戴冠2个月	戴冠3个月	F（X）	P
纯钛组	8.7±1.5	8.3±0.6	7.0±2.7	8.0±1.0	8.0±2.0	0.38	0.8169
钴铬钼合金组	9.0±1.0	8.0±1.0	9.3±0.6	9.0±2.7	9.3±2.1	0.27	0.8875
镍铬合金组	7.3±1.2	20.7±2.1	23.3±2.1	34.7±1.5	35.3±4.7	55.72	0.0001
空白对照组	7.7±1.5	7.7±0.6	7.3±1.5	6.7±2.1	7.7±0.6	0.76	0.5792

组别	戴冠前	戴冠2周	戴冠1个月	戴冠2个月	戴冠3个月	F（X）	P
纯钛组	8.7±1.5	8.3±0.6	7.0±2.7	8.0±1.0	8.0±2.0	0.38	0.8169
钴铬钼合金组	9.0±1.0	8.0±1.0	9.3±0.6	9.0±2.7	9.3±2.1	0.27	0.8875
镍铬合金组	7.3±1.2	20.7±2.1	23.3±2.1	34.7±1.5	35.3±4.7	55.72	0.0001
空白对照组	7.7±1.5	7.7±0.6	7.3±1.5	6.7±2.1	7.7±0.6	0.76	0.5792

[1]	Min Wang, Tao Cheng, Menghu Han, Shaohua Liang, Wen Wang. Comparative study of enhanced repair of huge rotator cuff tear with allograft acellular dermal patch [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2024, 18(06): 702-708.
[2]	Xiaobo Zhang, Te Ba, Ruijuan Huang, Hongyu Wang. Research progress on the mechanism of mesenchymal stem cell-derived exosomes on improving acute lung injury [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2025, 20(01): 81-85.
[3]	Fanrun Zeng, Yongyong Lin, Jun Wang. Advances in the mechanisms of mesenchymal stem cell-derived exosomes promoting wound angiogenesis [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2025, 20(01): 86-89.
[4]	Zhizhao Zhang, Rui Wang, Pingping Gao, Chengfang Wang, Cheng Wang, Xiaowei Qi. Relationship between DNMT3B and prognosis of breast cancer and its biological mechanism [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(06): 624-629.
[5]	Lingyan Wang, Chunhui Gao, Xueyuan Feng, Xinmiao Cui, Huan Liu, Wenming Zhao, Jinku Zhang. Application of circulating tumor cells in neoadjuvant and postoperative adjuvant therapy for breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(06): 630-633.
[6]	Qian Yu, Qingchao Cui, Yihui Fan, Yao Yao. Extracellular vesicles derived from Schwann cells promote pulp regeneration through the Wnt/β-catenin signaling pathway [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2025, 15(01): 1-11.
[7]	Yang Liu, Hong Zheng, Bihong Sun, Qian Yuan, Pei Yang, Haiying Guo, Yuhong Li. Visualization analysis of hair follicle stem cells in the field of hair loss based on CiteSpace [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2025, 15(01): 30-40.
[8]	Lin Xu, Dongsheng Yao, Yijing Zhou, Di Huang. LncRNA NEAT1/miR-129-5p axis was involved in LPS-induced renal tubular epithelial cell injury through regulating Wnt/β-catenin pathway activation [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2025, 15(01): 41-50.
[9]	Xinyi Zhuo, Yuchong Zhu, Junquan Liu, Yuqin Liao. Advances in the application of mesenchymal stem cells-derived nanovesicle in various diseases [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2025, 15(01): 57-62.
[10]	Min Zhang, Jianhua Zhu, Yafang Miao, Jinrong Guo. Inhibitory effect and mechanism of sarsasapogenin on hepatocellular carcinoma HepG2 cells [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(06): 328-335.
[11]	Jiangbo Ren, Li Li, Ping Wang. Blocking PI3K/Akt signal pathway promotes FoxA2 low-expressing liver progenitors to respond to differentiation reagent and differentiate towards hepatocytes [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(06): 336-343.
[12]	Hongxing Fu, Zhikai Wang, Guilin Xie, Juanjuan Cai, Wei Yang, Sheng Yan. Advances in mesenchymal stem cells promoting the efficacy of islet transplantation [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(06): 351-360.
[13]	Dawei Wang, Yafei Lu, Shaohua Huangfu, Yuting Chen, Ao Chen, Bin Jiang. Advances in immune modulating mechanisms of mesenchymal stem cells in promoting wound healing [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(06): 361-366.
[14]	Yuanyuan Yuan, Leqi Yue, Huaxing Zhang, Yan Wu, Quanhai Li. Advances in the distribution in lung tissue and therapeutic mechanisms of mesenchymal stem cells in respiratory system diseases models [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(06): 374-381.
[15]	Jun Chen, E-wen Tu, Zhao Wang. Diagnostic characteristics of mitochondrial encephalomyopathy caused by multiple deletions of mitochondrial DNA and literature review [J]. Chinese Journal of Diagnostics(Electronic Edition), 2025, 13(01): 59-64.

Please choose a citation manager

Content to export