血管内皮生长因子和骨形成蛋白2诱导犬恒牙原位牙髓再生

doi:10.3877/cma.j.issn.1674-1366.2016.01.002

中华口腔医学研究杂志(电子版) ›› 2016, Vol. 10 ›› Issue (01) : 5 -10. doi: 10.3877/cma.j.issn.1674-1366.2016.01.002

所属专题：文献；

基础研究

血管内皮生长因子和骨形成蛋白2诱导犬恒牙原位牙髓再生

周俊¹, 王兆晶¹, 陈文瑨¹, 陈文霞¹^,^†(

)

^1. 530021　南宁，广西医科大学附属口腔医院牙体牙髓科

收稿日期:2015-11-13 出版日期:2016-02-01
通信作者: 陈文霞
基金资助:
国家自然科学基金(地区科学基金项目，81160133)

Regeneration of dental pulp tissue in mature dog teeth with apical periodontitis using vascular endothelial growth factor and bone morphogenetic protein 2

Jun Zhou¹, Zhaojing Wang¹, Wenjin Chen¹, Wenxia Chen¹^,^†()

^1. Department of Operative Dentistry and Endodontics, College & Hospital of Stomatology Guangxi Medical University, Nanning 530021, China

Received:2015-11-13 Published:2016-02-01
Corresponding author: Wenxia Chen
About author:
Corresponding author: Chen Wenxia, Email: angelaxiacw@163.com

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目的

通过选择犬根尖孔发育完成的恒牙建立根尖周炎模型，探索血管内皮生长因子（VEGF）和骨形态生成蛋白2（BMP2）诱导原位牙髓再生的可能性。

方法

2只10~ 12月龄的杂种犬，选择根尖孔发育完成的14颗恒前牙建立根尖周炎模型，分别将VEGF（VEGF组）、BMP2（BMP2组）单独和VEGF+BMP2联合（VEGF+BMP2组）与水凝胶复合植入感染控制后的根管腔内，对照组仅植入水凝胶。8周后组织学观察根管内组织再生情况。

结果

植入8周后，VEGF组和VEGF+BMP2组根管腔内可见含有大量成纤维样细胞和血管的新生组织形成；而BMP2组和对照组根管腔内见均质状物质，未见细胞、血管形成。

结论

VEGF或VEGF+BMP2复合水凝胶支架可以诱导犬根尖发育成熟的根尖周炎患牙在根管腔内生成含有血管的疏松结缔组织。

关键词: 血管内皮生长因子类, 骨形态生成蛋白2, 牙髓, 再生, 根尖周炎

Objective

To investigate the feasibility of dental pulp regeneration in mature teeth with apical periodontitis on situ using vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP2) .

Methods

Apical periodontitis model was established in 14 mature anterior teeth in 2 dogs (10-12 months) . The disinfected root canals were filled with peptide hydrogel scaffold composited with different cytokines: VEGF group, BMP2 group, VEGF+BMP2 group and a control group (without cytokines) . Eight weeks after the operation, a histological observation was undertaken to evaluate the regeneration tissue in the root canals.

Results

Eight weeks after the operation, newly formed vascularized connective tissue were found in the root canals which filled with VEGF and VEGF+BMP2. No cells and vessels were observed in the root canals in BMP2 group and control group.

Conclusion

VEGF alone or combinated with BMP2 can induce pulp-like tissue regeneration within the root canals of the mature teeth with apical periodontitis.

Key words: Vascular endothelial growth factors, Bone morphogenetic protein 2, Dental pulp, Regeneration, Periapical periodontitis

图1 实验犬恒牙根尖周炎建模X线表现

图2 根管内植入VEGF+BMP2 2个月后组织学表现（苏木精-伊红）

图3 根管内植入VEGF 2个月后组织学表现（苏木精-伊红）

图5 根管内植入水凝胶2个月后组织学表现（苏木精-伊红）

图6 正常犬牙髓的组织学表现（苏木精-伊红）

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