目的

初步探讨自行研制的新型引导组织再生膜材料（仿生型改性胶原膜）应用于动物引导牙周组织再生的能力。

方法

通过模拟牙周炎骨缺损形态在Beagle 犬双侧下颌第三、四前磨牙颊侧制作3 个大小为5 mm×5 mm 的急性二壁骨袋的骨缺损模型，深达牙面。采用自体对照方法观察牙周组织再生情况，骨缺损区随机分为3 组：胶原膜组、聚四氟乙烯（e-PTFE）膜组、空白对照组，共5 只Beagle 犬、14 个实验部位。 术后2、4、8、12 周将分别进行大体标本观察、组织学观察、锥形束计算机体层摄影术（CBCT）扫描并重建、扫描电镜Ca、P 元素微区定量测定。

结果

该仿生型改性胶原膜具有良好的骨引导和暴露后抗感染能力。 术后12 周，仿生型改性胶原膜组的新生骨高度、体积与e-PTFE 膜组对比差异无统计学意义；其引导的新生骨钙磷比值（Ca/P 值）高于空白组及e-PTFE 膜组。

结论

动物实验显示，该新型仿生型改性胶原膜具有良好的提高牙周骨再生能力。

Objective

To introduce our research on a new modified bionic collagen membrane，which will be used for animal periodontal tissue regeneration in our experiment and discussed about its ability to guide tissue regeneration， after our former work.

Methods

Three acute two-wall intrabony defects （5 mm × 5 mm， deep enough to expose the teeth surface） were created randomly on the buccal aspects of three bilateral lower third and fourth premolars of Beagle dog to imitate human periodontitis'alveolar bone defects. Autologous-control groups were adopted in our experiment， and the specimens were classified into three groups randomly： the collagen membrane group， the e-PTFE group and the blank-controlled group. There were 5 Beagle dogs and 14 specimens. At 2， 4， 8， 12 weeks， the following researches were undertaken after one dog was executed： general specimens observation，histological observation， the three-dimensional computed tomography analysis and reconstruction， the scanning electron microscopy （SEM） observation and energy-dispersive X-ray of Ca/P.

Results

The new modified bionic collagen membrane has a good ability to guide bone regeneration and is resistant to infection after exposion. At 12 weeks， new bone heights， volumes of the collagen membrane group and the e-PTFE group had no statistical difference； the Ca/P molar ratio of collagen membrane group was higher than other groups.

Conclusion

The animal experiment shows that the new modified bionic collagen membrane can enhance periodontal bone regeneration effectively.