Condyle chondrocytes regeneration promoted by exosomes from bone marrow mesenchymal stem cells

doi:10.3877/cma.j.issn.1674-1366.2021.04.003

Abstract

Abstract:

Objective

The aim of this study was to determine the effects of exosomes from rat bone marrow mesenchymal stem cells (BMSCs) on condyle chondrocytes.

Methods

Exosomes (Exos) was extracted from rat BMSCs to culture rat condyle chondrocytes. Cell counting kit-8 (CCK-8) was applied to analyze cell proliferation of condyle chondrocytes of BMSC-Exos group and control group (L-DMEM) . Cell cycle progression was analyzed by flow cytometry. RT-PCR was applied to measure chondrogenic gene expression levels[Collagen I (Col I) and Col II) ]. Comparisons between groups were performed with independent-samples T test.

Results

The results of CCK-8 showed that the cell proliferation activity of BMSC-Exos group was 4.72 ± 0.58, which was significantly higher than that of the control group, 2.91 ± 0.17 (t = 16.13, P<0.001) . The results of flow cytometry showed that Exos could promote condyle chondrocytes transformed from G1 to G2. The results of RT-PCR indicated that the expression increment of type Col I gene in BMSC-Exos group at day 7 was 2.85 ± 0.14, which was higher than that in the control group 1.00 ± 0.17 (t = 13.41, P<0.001) . Besides, the increment of expression of Col I gene in BMSC-Exos group I on day 14 was 5.34 ± 0.17, which was higher than that in the control group 3.14 ± 0.10 (t = 14.25, P<0.001) . Meanwhile, the increment of Col II gene expression in the experimental group was 1.77 ± 0.13, which was higher than that in the control group 1.35 ± 0.18 at the 14th day (t = 3.52, P = 0.041) .

Conclusions

Exosomes from rat bone marrow mesenchymal stem cells can promote the condyle chondrocytes proliferation. Besides, it can upregulate the expression of Col I and Col II in condyle chondrocytes.

Key words: Rats, Bone marrow cells, Mesenchymal stem cell, Mandibular condyle, Chondrocytes, Exosomes, Cell proliferation, Temporomandibular joint, Osteoarthropathy

Chao Xing, Lingqiao Xu, Wenting Liao, Yangpeng Sun, Zhongtai Ye, Zhiguang Zhang. Condyle chondrocytes regeneration promoted by exosomes from bone marrow mesenchymal stem cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2021, 15(04): 207-214.

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References 18

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目的基因	引物序列	产物长度（bp）	基因库登记号
ColⅠ	F:5′-CTACAGCACGCTTGTGGATG-3′	195	Z_78279
	R:5′-CAGATTGGGATGGAGGGAGT-3′
ColⅡ	F:5′-AGAACTGGTGGAGCAGCAAGA-3′	124	NM_012929
	R:5′-ATCTGGACGTTAGCGGTGTTG-3′
GAPDH	F:5′-GGCACAGTCAAGGCTGAGAATG-3′	143	NM_0170084
	R:5′-ATGGTGGTGAAGACGCCAGTA-3′

目的基因	引物序列	产物长度（bp）	基因库登记号
ColⅠ	F:5′-CTACAGCACGCTTGTGGATG-3′	195	Z_78279
	R:5′-CAGATTGGGATGGAGGGAGT-3′
ColⅡ	F:5′-AGAACTGGTGGAGCAGCAAGA-3′	124	NM_012929
	R:5′-ATCTGGACGTTAGCGGTGTTG-3′
GAPDH	F:5′-GGCACAGTCAAGGCTGAGAATG-3′	143	NM_0170084
	R:5′-ATGGTGGTGAAGACGCCAGTA-3′

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