Expression and role of platelet-derived growth factor D in tongue squamous cell carcinoma

doi:10.3877/cma.j.issn.1674-1366.2019.03.004

Abstract

Abstract:

Objective

To explore the role of platelet-derived growth factor D (PDGF-D) in human tongue squamous cell carcinoma (TSCC) .

Methods

In total, 127 human tongue carcinoma specimens and 15 adjacent tumor specimens were tested for PDGF-D with immunohistochemical approaches. T-test was used to analyze the relationship between the expression of PDGF-D and the age, gender, TNM stage, clinical stage of patients. Survival analysis was conducted in a proportional hazards model. PDGF-D overexpressed and knockdown cells were constructed. The proliferative, migrative, and invasive ability of TSCC cells was assessed in vitro. Epithelial-mesenchymal transition (EMT) related proteins were tested.

Results

Immunohistochemical results demonstrated that the expression of PDGF-D was higher in cancer tissue than that of normal tissue (P<0.001) . Chi-square test showed that the expression of PDGF-D was related to the TNM stage, clinical stage and lymph node metastasis of TSCC patients. Survival analysis showed that patients with low PDGF-D expression were expected to have longer survival time (P=0.0446) . PDGF-D may act as an independent risk factor (HR=2.643, P<0.05) . PDGF-D overexpressed cells formed more colonies in the colony forming test (P<0.001) , and showed more penetrated cells in the transwell migration (P=0.044) and invasion (P<0.001) tests. By contrast, PDGF-D knocked down cells formed less colonies in the colony forming test (P=0.026) , and showed less penetrated cells in the transwell migration (P<0.001) and invasion (P<0.001) tests. Western blot showed that the epithelial protein marker (E-cad) was depressed while the expression of mesenchymal markers (N-cad, Vimentin) was upregulated in PDGF-D overexpressed cells. The effect of knocking down PDGF-D on TSCC cell and EMT was in the reverse way.

Conclusions

The expression of PDGF-D was strongly expressed in tongue carcinoma and correlated with its prognosis. PDGF-D enhanced the proliferation, migration, and invasion of TSCC cells. Statistically, PDGF-D may act as an independent prognostic factor for TSCC.

Key words: Platelet-derived growth factor, Carcinoma, squamous cell, Tongue, Neoplasm metastasis, Epithelial-mesenchymal transition

Quan Wan, Jiuyang Jiao, Zhiyuan Lu, Chaobin Pan. Expression and role of platelet-derived growth factor D in tongue squamous cell carcinoma[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(03): 151-157.

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Figures/Tables 9

References 13

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因素		低表达	高表达	χ²值	P值
年龄		?	?	?	?
?	≤55	34	28	0.211	0.646
?	>55	33	32	?	?
性别		?	?	?	?
?	男	41	39	0.197	0.657
?	女	26	21	?	?
TNM分期		?	?	?	?
?	T1+T2	52	33	7.312	0.007
?	T3+T4	15	27	?	?
淋巴结转移		?	?	?	?
?	LN+	18	32	9.290	0.002
?	LN-	49	28	?	?
临床分期		?	?	?	?
?	Ⅰ+Ⅱ	33	40	3.927	0.048
?	Ⅲ+Ⅳ	34	20	?	?
病理分化		?	?	?	?
?	分化较好	47	27	8.233	0.004
?	中-较差	20	33	?	?

因素		低表达	高表达	χ²值	P值
年龄		?	?	?	?
?	≤55	34	28	0.211	0.646
?	>55	33	32	?	?
性别		?	?	?	?
?	男	41	39	0.197	0.657
?	女	26	21	?	?
TNM分期		?	?	?	?
?	T1+T2	52	33	7.312	0.007
?	T3+T4	15	27	?	?
淋巴结转移		?	?	?	?
?	LN+	18	32	9.290	0.002
?	LN-	49	28	?	?
临床分期		?	?	?	?
?	Ⅰ+Ⅱ	33	40	3.927	0.048
?	Ⅲ+Ⅳ	34	20	?	?
病理分化		?	?	?	?
?	分化较好	47	27	8.233	0.004
?	中-较差	20	33	?	?

因素	单因素分析		多因素分析
因素	P值	HR	95% CI	P值
年龄（≤55 vs>55，岁）	0.058	0.602	0.323，1.123	0.111
性别（男vs女）	0.697	0.765	0.424，1.378	0.372
TNM分期（T1+T2 vs T3+T4）	<0.001	0.422	0.143，1.243	0.118
淋巴结转移（LN⁺ vs LN^-）	0.010	0.507	0.200，1.280	0.150
临床分期（Ⅰ+Ⅱ vs Ⅲ+Ⅳ）	<0.001	0.435	0.112，1.690	0.229
PDGF-D表达（低vs高）	<0.001	2.643	1.104，6.887	0.047

因素	单因素分析		多因素分析
因素	P值	HR	95% CI	P值
年龄（≤55 vs>55，岁）	0.058	0.602	0.323，1.123	0.111
性别（男vs女）	0.697	0.765	0.424，1.378	0.372
TNM分期（T1+T2 vs T3+T4）	<0.001	0.422	0.143，1.243	0.118
淋巴结转移（LN⁺ vs LN^-）	0.010	0.507	0.200，1.280	0.150
临床分期（Ⅰ+Ⅱ vs Ⅲ+Ⅳ）	<0.001	0.435	0.112，1.690	0.229
PDGF-D表达（低vs高）	<0.001	2.643	1.104，6.887	0.047

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