The research progress of human organoids and the prospects of application in stomatology

doi:10.3877/cma.j.issn.1674-1366.2019.02.001

Abstract

Abstract:

The organoid is a multicellular mass with the ability of self-renewal and self-organization in artificial extracellular matrix, which is constructed by three-dimensional culture in vitro, and it maintains the physiological structure and function of its source tissues. As a new research model, the organoid has the advantages of both cell lines and animal models. The easily material construction, high culturing efficiency and short time-consuming make it good application prospects in disease research model construction, clinical drug sensitivity test and regenerative medicine application. The organoid models can be divided into three types of cell sources: embryonic stem cells/induced pluripotent stem cells, adult stem cells and cancer cells. In this review, organoid from three cell sources will be elaborated separately, and mainly focused on the current status of human organoid research progress and the prospect of application in stomatology.

Key words: Organoid, Embryonic stem cells, Induced pluripotent stem cells, Adult stem cells, Neoplasms

Rukeng Tan, Runling Zeng, Zhuoran Wang, Meng Xu. The research progress of human organoids and the prospects of application in stomatology[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(02): 65-70.

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References 35

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组织类型	组织来源	胚层分化培养基	器官分化培养基	类器官生长培养基
肠^[5]	人源胚胎干细胞和诱导性多能干细胞	含有Activin A的RPMI 1640培养基	含有成纤维细胞生长因子4、 CHIR99021的RPMI 1640培养基	含有表皮细胞生长因子、头蛋白、R-spondin1的高级DMEM/F12培养基、Matrigel
胃^[6]	人源胚胎干细胞和诱导性多能干细胞系	含有Activin A、BMP4的RPMI 1640培养基	WNT3A、CHIR99021、成纤维细胞生长因子4、头蛋白、维甲酸的RPMI 1640培养基	含有表皮细胞生长因子、头蛋白、维甲酸的高级DMEM/F12培养基、 Matrigel
肝脏^[7]	人源胚胎干细胞和诱导性多能干细胞系	含有Activin A、BMP4、CHIR 99021、成纤维细胞生长因子2、 LY294002的DMEM/F12培养基	含有成纤维细胞生长因子10、 BMP4的RPMI及B27培养基	含有肝细胞生长因子、抑瘤素M的高级DMEM/F12培养基、Matrigel
脑^[8]	人源胚胎干细胞和诱导性多能干细胞系	含有endo-IWR1、LDN-193189、 SB431542、β-巯基乙醇的KSR培养基	含有NEAA、BDNF、GDNF的neurobasal-type differentiation培养基、Matrigel	DMEM/F12培养基、Matrigel
肾^[9]	人源胚胎干细胞和诱导性多能干细胞系	含有Activin的高级RPMI培养基	含有成纤维细胞生长因子9、 CHIR99021的高级RPMI培养基	含有CHIR99021、头蛋白、成纤维细胞生长因子9的高级RPMI 1640培养基
肺^[10]	人源胚胎干细胞系	含有Activin A、BMP4的DMEM/ F12培养基	含有成纤维细胞生长因子7，CHIR - 99021、维甲酸的DMEM/ F12培养基	DMEM/F12培养基、Matrigel
乳腺^[11]	人源诱导性多能干细胞系	complete MammoCult培养基	含有成纤维细胞生长因子10、肝细胞生长因子、催乳素的EpiCult B培养基、Matrigel/CollagenⅠ	含有成纤维细胞生长因子10、肝细胞生长因子的EpiCult B培养基、 Matrigel/CollagenⅠ

组织类型	组织来源	胚层分化培养基	器官分化培养基	类器官生长培养基
肠^[5]	人源胚胎干细胞和诱导性多能干细胞	含有Activin A的RPMI 1640培养基	含有成纤维细胞生长因子4、 CHIR99021的RPMI 1640培养基	含有表皮细胞生长因子、头蛋白、R-spondin1的高级DMEM/F12培养基、Matrigel
胃^[6]	人源胚胎干细胞和诱导性多能干细胞系	含有Activin A、BMP4的RPMI 1640培养基	WNT3A、CHIR99021、成纤维细胞生长因子4、头蛋白、维甲酸的RPMI 1640培养基	含有表皮细胞生长因子、头蛋白、维甲酸的高级DMEM/F12培养基、 Matrigel
肝脏^[7]	人源胚胎干细胞和诱导性多能干细胞系	含有Activin A、BMP4、CHIR 99021、成纤维细胞生长因子2、 LY294002的DMEM/F12培养基	含有成纤维细胞生长因子10、 BMP4的RPMI及B27培养基	含有肝细胞生长因子、抑瘤素M的高级DMEM/F12培养基、Matrigel
脑^[8]	人源胚胎干细胞和诱导性多能干细胞系	含有endo-IWR1、LDN-193189、 SB431542、β-巯基乙醇的KSR培养基	含有NEAA、BDNF、GDNF的neurobasal-type differentiation培养基、Matrigel	DMEM/F12培养基、Matrigel
肾^[9]	人源胚胎干细胞和诱导性多能干细胞系	含有Activin的高级RPMI培养基	含有成纤维细胞生长因子9、 CHIR99021的高级RPMI培养基	含有CHIR99021、头蛋白、成纤维细胞生长因子9的高级RPMI 1640培养基
肺^[10]	人源胚胎干细胞系	含有Activin A、BMP4的DMEM/ F12培养基	含有成纤维细胞生长因子7，CHIR - 99021、维甲酸的DMEM/ F12培养基	DMEM/F12培养基、Matrigel
乳腺^[11]	人源诱导性多能干细胞系	complete MammoCult培养基	含有成纤维细胞生长因子10、肝细胞生长因子、催乳素的EpiCult B培养基、Matrigel/CollagenⅠ	含有成纤维细胞生长因子10、肝细胞生长因子的EpiCult B培养基、 Matrigel/CollagenⅠ

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