The roles of dental pulp cell derived exosomes in human umbilical vein endothelial cells migration

doi:10.3877/cma.j.issn.1674-1366.2018.01.003

Abstract

Abstract:

Objective

To investigate the effect of dental pulp cell derived exosomes (DPC-Exos) on human umbilical vein endothelial cells (HUVECs) migration in vitro.

Methods

Exosomes were isolated from the supernatant of DPCs cell culture by ultracentrifugation and characterized by transmission electron microscope (TEM) , western blotting. The effect of DPC-Exos on HUVECs migration was examined by wounding healing assay and transwell assay in vitro. Two independent samples t test was used to statistically analyze the migration rate and number of migration cells by SPSS 20.0 software.

Results

The bi-layer membrane and "saucer-like" appearance of DPC-Exos were examined by transmission electron microscopy (TEM) . Western blotting revealed that CD63 was expressed in the DPC-Exos. Wound-healing assay showed significantly reduced migration ability by 26% in HUVECs after treated with DPC-Exos (t= 6.534, P<0.001) . Transwell assay results indicated that DPC-Exos treatment could significantly reduced migration ability of HUVECs by 32% (t= 5.846, P<0.001) .

Conclusion

DPC-Exos could significantly suppress the migration of HUVECs.

Key words: Dental pulp, Exosomes, Cell migration assays, Angiogenesis

Xuehong Xian, Qimei Gong, Hongwei Jiang. The roles of dental pulp cell derived exosomes in human umbilical vein endothelial cells migration[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2018, 12(01): 14-18.

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Figures/Tables 4

References 14

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