Study on Dexamethasone regulation of dentin matrix protein 1 expression in osteoblast

doi:10.3877/cma.j.issn.1674-1366.2017.02.003

Abstract

Abstract:

Objective

To analyze the expression changes of dentin matrix protein 1 (DMP1) regulated by Dexamethasone (Dex) in osteoblast cells.

Methods

Using the quantitative real-time PCR reaction technology to analyze the changes of DMP1 gene expression in MC3T3-E1 cell at various times points under Dex treatment with different concentrations. Then the difference between experimental groups and control groups in 48 h and various concentrations was compared by the data from real-time PCR using One-Way ANOVA statistical method. By means of the western-blot technology, the protein expression changes of DMP1 at various hours in MC3T3-E1 cell under 10^-6 g/L Dex treatment were analyzed.

Results

In MC3T3-E1 cells treated with different concentrations Dex, the DMP1 gene expressions were significantly reduced at 24, 48 and 72 h compared with 0 h (P<0.05) , and 48 h was decreased significantly compared with 24 and 72 h (P<0.05) ; in 48 h the 10^-6 g/L group was decreased significantly compared with 10^-7 g/L (P<0.05) and with no significant difference compared with 10^-5 g/L (P<0.05) . In MC3T3-E1 cells under 10^-6 g/L Dex treatment, the DMP1 protein expression of various time points were lower than the baseline (0 h) .

Conclusions

The expression of DMP1 was suppressed by Dexamethasone in osteoblast MC3T3-E1, which suggested that Dex may influence the development of bone cells by regulate the expression of DMP1.

Key words: Dexamethasone, Gene expression regulation, Dentin matrix protein 1

Xin Xia, Yi Ruan, Jiangyuan Chen, Boya Li, Xiangbo Kong, Hong Wu. Study on Dexamethasone regulation of dentin matrix protein 1 expression in osteoblast[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2017, 11(02): 81-85.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2017.02.003

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2017/V11/I02/81

Figures/Tables 6

References 19

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地塞米松浓度(g/L)	DMP1相对基因表达水平
地塞米松浓度(g/L)	0 h	24 h	48 h	72 h
10^-7	1.000 ± 0.004	0.519 ± 0.004	0.361 ± 0.014	0.664 ± 0.023
10^-6	1.000 ± 0.038	0.228 ± 0.026	0.134 ± 0.009	0.329 ± 0.016
10^-5	1.000 ± 0.018	0.208 ± 0.021	0.106 ± 0.027	0.285 ± 0.010

地塞米松浓度(g/L)	DMP1相对基因表达水平
地塞米松浓度(g/L)	0 h	24 h	48 h	72 h
10^-7	1.000 ± 0.004	0.519 ± 0.004	0.361 ± 0.014	0.664 ± 0.023
10^-6	1.000 ± 0.038	0.228 ± 0.026	0.134 ± 0.009	0.329 ± 0.016
10^-5	1.000 ± 0.018	0.208 ± 0.021	0.106 ± 0.027	0.285 ± 0.010

地塞米松浓度(g/L)		均差平方和	自由度均方差	F值	P值
10^-5		?	?	702.627	0.000
?	组间	0.641	3 0.214	?	?
?	组内	0.002	8 0.000	?	?
10^-6		?	?	894.759	0.000
?	组间	1.397	3 0.466	?	?
?	组内	0.004	8 0.001	?	?
10^-7		?	?	2283.382	0.000
?	组间	1.503	3 0.501	?	?
?	组内	0.002	8 0.000	?	?

地塞米松浓度(g/L)		均差平方和	自由度均方差	F值	P值
10^-5		?	?	702.627	0.000
?	组间	0.641	3 0.214	?	?
?	组内	0.002	8 0.000	?	?
10^-6		?	?	894.759	0.000
?	组间	1.397	3 0.466	?	?
?	组内	0.004	8 0.001	?	?
10^-7		?	?	2283.382	0.000
?	组间	1.503	3 0.501	?	?
?	组内	0.002	8 0.000	?	?

地塞米松浓度(g/L)	组间比较		P值
10^-5	0 h	VS 24 h	0.002
?	?	VS 48 h	0.000
?	?	VS 72 h	0.000
?	24 h	VS 48 h	0.005
?	?	VS 72 h	0.022
?	48 h	VS 72 h	0.001
10^-6	0 h	VS 24 h	0.000
?	?	VS 48 h	0.001
?	?	VS 72 h	0.001
?	24 h	VS 48 h	0.014
?	?	VS 72 h	0.006
?	48 h	VS 72 h	0.001
10^-7	0 h	VS 24 h	0.000
?	?	VS 48 h	0.000
?	?	VS 72 h	0.000
?	24 h	VS 48 h	0.045
?	?	VS 72 h	0.523
?	48 h	VS 72 h	0.000

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