Hypoxia induced autophagy and promotes the migration and invasion in tongue squamous cell carcinoma

doi:10.3877/cma.j.issn.1674-1366.2016.06.002

Abstract

Abstract:

Objective

To investigate the effect of hypoxia on autophagy and migration of tongue squamous cell carcinoma (TSCC) cell line.

Methods

UM1 cells were cultured in hypoxia incubator containing 1% oxygen concentration. Western blot was performed to examine the expression of HIF-1α, Beclin-1, ATG5 and LC3 following 3, 6, 9, 12 and 24 h of treatment of hypoxia. Wounding healing was used to evaluate cell migration. Transwell assay was carried out to assess cell invasion. Statistical analysis was performed using SPSS 13.0 software.

Results

After, The expression level of autophagic protein LC3Ⅱ (1.206 ± 0.053) in the hypoxia treatment group was higher than control group (0.527 ± 0.055) and both showed statistically significant difference (t= 12.96, P<0.001) ; the expression level of Beclin-1 (1.151 ± 0.078) in the hypoxia treatment group was higher than control group (0.775 ± 0.062) , and both showed a statistically significant difference (t= 6.736, P= 0.001) ; the expression level of ATG5 (1.231 ± 0.06) in the hypoxia treatment group was higher than control group (0.711 ± 0.052) , was and both showed a statistically significant difference (t= 7.834, P= 0.001) . The migration ability of TSCCUM1 (0.349 ± 0.024) was improved under hypoxia in comparison to control groups (0.788 ± 0.037) (t= 9.918, P= 0.001) . The invasion ability of TSCCUM1 (23 ± 2) was upregulated under hypoxia in comparison to control groups (71 ± 4) (t= 9.528, P= 0.001) .

Conclusion

Hypoxia can improve autophagic activity levels and promotes the migration and invasion in tongue squamous cell carcinoma cell line.

Key words: Tongue, Carcinoma, squamous cell, Hypoxia, Autophagy, Migration, Invasion

Guanhui Chen, Cheng Wang, Junquan Weng, Jianfeng Liang, Wenqing Li, Yue Zhu, Jinsong Hou. Hypoxia induced autophagy and promotes the migration and invasion in tongue squamous cell carcinoma[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(06): 372-376.

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Figures/Tables 5

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