The effect of miR-181a on invasion and migration of salivary adenoid cystic carcinoma

doi:10.3877/cma.j.issn.1674-1366.2014.06.002

Abstract

Abstract:

Objective

To screen microRNA（miRNA） related to the cell invasion and metastasis in salivary adenoid cystic carcinoma （SACC） cell lines and investigate the effect of miR-181a in the cell invasion and migration in SACC.

Methods

A paired SACC cell lines（SACC-LM/SACC-83） with different cell invasive and migrative ability were chosen. MiRNA array was performed to analyze the different expression of miRNAs in the paired SACC cell lines and identify miRNAs related to the cell invasion and metastasis. Then SACC-LM and SACC-83 were transfected with miR-181a mimics and miR-181a LNA respectively. Wound healing assay and transwell assay were used to observe the changes of the cell migration and invasion ability after transfection. Data were analyzed using the Statistical Package for the Social Science （SPSS）， Version 17.0. For all statistical analyses， P ＜0.05 was considered statistically significant.

Results

MiRNA array showed that the expression of miR-181a in SACC-LM cells was lower than SACC-83 cells. Wound healing assay and transwell assay showed that ectopic transfection of the miR-181a mimics into the SACC-LM cells led to inhibition in the cell invasion and migration （t=-5.235，P ＜0.05）. When the SACC-83 cells were treated with miR-181a LNA， an improvement in the cell invasive and migrative ability was observed （t=7.713，P ＜0.05）.

Conclusions

There are various miRNAs expressing differently in SACC cell lines with divergent invasive and migrative ability. Underexpression of miR-181a could enhance the cell invasive and migrative ability of SACC cells.

Key words: MicroRNA, MiR-181a, Salivary adenoid cystic carcinoma, Invasion, Migration

Qianting He, Dan Chen, Zhonghua Liu, Tingting Zhao, Anxun Wang. The effect of miR-181a on invasion and migration of salivary adenoid cystic carcinoma[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(06): 447-453.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2014.06.002

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2014/V8/I06/447

Figures/Tables 8

References 13

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细胞株	A ₂₆₀	A ₂₈₀	A ₂₆₀/A₂₈₀	总量（μg）
SACC-83	0.364	0.172	2.10	29.12
SACC-LM	0.178	0.085	2.01	14.24

细胞株	A ₂₆₀	A ₂₈₀	A ₂₆₀/A₂₈₀	总量（μg）
SACC-83	0.364	0.172	2.10	29.12
SACC-LM	0.178	0.085	2.01	14.24

miRNA	SACC-83组均数	SACC-LM组均数	校正SACC-83组	校正SACC-LM组	SACC-LM/SACC-83
1529-shuf-has-mir-93	478	656	6.966	11.960	1.72
1532-rev-mir-150	794	1202	7.130	13.830	1.94
1565-shuf-mir-34c	345	254	8.107	8.313	1.03
hsa-miR-105	494	200	11.130	6.183	0.56
hsa-miR-135a	578	407	9.429	8.441	0.90
hsa-miR-148a	365	116	10.950	5.389	0.49
hsa-miR-15b	340	120	10.310	5.680	0.55
hsa-miR-16	449	145	11.700	5.461	0.47
hsa-miR-17	456	301	9.122	7.978	0.87
hsa-miR-181a	564	162	13.080	5.189	0.40
hsa-miR-181b	405	131	11.290	5.450	0.48
hsa-miR-181c	295	102	9.822	5.626	0.57
hsa-miR-181d	341	133	10.000	5.975	0.60
hsa-miR-185	656	304	11.410	6.787	0.59
hsa-miR-186	314	128	9.567	6.113	0.64
hsa-miR-202	1306	1	114.200	0.235	0.00
hsa-miR-21	615	217	12.440	5.822	0.47
hsa-miR-214	984	454	12.350	7.132	0.58
hsa-miR-216b	238	289	6.071	10.630	1.75
hsa-miR-221	298	110	9.681	5.805	0.60
hsa-miR-25	1047	990	9.072	11.000	1.21
hsa-miR-27b	512	489	8.157	9.858	1.21
hsa-miR-296-3p	2170	1793	10.580	11.320	1.07
hsa-miR-29a	390	142	10.710	5.796	0.54
hsa-miR-30c-2	362	95	11.570	4.906	0.42
hsa-miR-335	448	358	8.460	8.835	1.04
hsa-miR-338-5p	351	4	29.980	0.911	0.03
hsa-miR-363	272	308	6.488	10.300	1.59
hsa-miR-383	403	313	8.318	8.629	1.04
hsa-miR-421	391	384	7.567	9.770	1.29
hsa-miR-452	545	417	9.010	8.784	0.97
hsa-miR-487a	421	411	7.725	9.795	1.27
hsa-miR-503	363	336	7.577	9.417	1.24
hsa-miR-510	369	195	9.282	7.011	0.76
hsa-miR-513-5p	620	403	9.885	8.131	0.82
hsa-miR-548a-5p	330	270	7.702	8.773	1.14
hsa-miR-548b-5p	371	311	7.907	8.940	1.13
hsa-miR-548c-3p	348	19	18.420	2.186	0.12
hsa-miR-568	365	293	7.988	8.733	1.09
hsa-miR-629	346	127	10.250	5.794	0.57
hsa-miR-630	928	960	8.586	11.370	1.32
hsa-miR-638	1111	759	10.530	9.196	0.87
hsa-miR-674	1857	860	13.350	7.954	0.60
hsa-miR-877	756	458	10.580	8.002	0.76
hsa-miR-890	455	1	55.080	0.354	0.01
hsa-miR-892b	265	339	6.174	10.960	1.78
hsa-miR-923	5209	5919	10.140	15.260	1.51
hsa-miR-93	354	235	8.470	7.884	0.93
hsa-miR-933	771	160	16.040	4.458	0.28
hsa-miR-944	539	1	62.050	0.332	0.01

miRNA	SACC-83组均数	SACC-LM组均数	校正SACC-83组	校正SACC-LM组	SACC-LM/SACC-83
1529-shuf-has-mir-93	478	656	6.966	11.960	1.72
1532-rev-mir-150	794	1202	7.130	13.830	1.94
1565-shuf-mir-34c	345	254	8.107	8.313	1.03
hsa-miR-105	494	200	11.130	6.183	0.56
hsa-miR-135a	578	407	9.429	8.441	0.90
hsa-miR-148a	365	116	10.950	5.389	0.49
hsa-miR-15b	340	120	10.310	5.680	0.55
hsa-miR-16	449	145	11.700	5.461	0.47
hsa-miR-17	456	301	9.122	7.978	0.87
hsa-miR-181a	564	162	13.080	5.189	0.40
hsa-miR-181b	405	131	11.290	5.450	0.48
hsa-miR-181c	295	102	9.822	5.626	0.57
hsa-miR-181d	341	133	10.000	5.975	0.60
hsa-miR-185	656	304	11.410	6.787	0.59
hsa-miR-186	314	128	9.567	6.113	0.64
hsa-miR-202	1306	1	114.200	0.235	0.00
hsa-miR-21	615	217	12.440	5.822	0.47
hsa-miR-214	984	454	12.350	7.132	0.58
hsa-miR-216b	238	289	6.071	10.630	1.75
hsa-miR-221	298	110	9.681	5.805	0.60
hsa-miR-25	1047	990	9.072	11.000	1.21
hsa-miR-27b	512	489	8.157	9.858	1.21
hsa-miR-296-3p	2170	1793	10.580	11.320	1.07
hsa-miR-29a	390	142	10.710	5.796	0.54
hsa-miR-30c-2	362	95	11.570	4.906	0.42
hsa-miR-335	448	358	8.460	8.835	1.04
hsa-miR-338-5p	351	4	29.980	0.911	0.03
hsa-miR-363	272	308	6.488	10.300	1.59
hsa-miR-383	403	313	8.318	8.629	1.04
hsa-miR-421	391	384	7.567	9.770	1.29
hsa-miR-452	545	417	9.010	8.784	0.97
hsa-miR-487a	421	411	7.725	9.795	1.27
hsa-miR-503	363	336	7.577	9.417	1.24
hsa-miR-510	369	195	9.282	7.011	0.76
hsa-miR-513-5p	620	403	9.885	8.131	0.82
hsa-miR-548a-5p	330	270	7.702	8.773	1.14
hsa-miR-548b-5p	371	311	7.907	8.940	1.13
hsa-miR-548c-3p	348	19	18.420	2.186	0.12
hsa-miR-568	365	293	7.988	8.733	1.09
hsa-miR-629	346	127	10.250	5.794	0.57
hsa-miR-630	928	960	8.586	11.370	1.32
hsa-miR-638	1111	759	10.530	9.196	0.87
hsa-miR-674	1857	860	13.350	7.954	0.60
hsa-miR-877	756	458	10.580	8.002	0.76
hsa-miR-890	455	1	55.080	0.354	0.01
hsa-miR-892b	265	339	6.174	10.960	1.78
hsa-miR-923	5209	5919	10.140	15.260	1.51
hsa-miR-93	354	235	8.470	7.884	0.93
hsa-miR-933	771	160	16.040	4.458	0.28
hsa-miR-944	539	1	62.050	0.332	0.01

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