Abstract:
Objective
To evaluate the in vivo dental pulp regeneration capacity of various concentration of the platelet-rich plasma （PRP）.
Methods
The tooth roots of minipigs were prepared by chemical methods. PRP was extracted by two-step centrifugation. The roots were divided into four groups according to the kinds of scaffold injected into the root canals （5 roots each group）： （1）control group， whole blood； （2）100% PRP； （3）50% PRP； （4）blank group： root fragments with empty canal space. The roots were implanted subcutaneously in nude mice. The animals were sacrificed after 5 weeks.The samples were taken for histological examination.
Results
The newly formed pulp-like tissues were observed in the roots which filled 50% PRP scaffolds in canals. The group of 100% PRP scaffolds were full of inflammatory cells.
Conclusions
It is feasible for the platelet-rich plasma with proper concentration as scaffolds to induce dental pulp regeneration in vivo.
Key words:
Platelet-rich plasma scaffolds,
Stem cell homing,
Dental pulp regeneration
Wei Wei, Yang Huang, Kangjing Li, Wenxia Chen. Platelet-rich plasma scaffolds induce dental pulp-like tissue regeneration in vivo[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(02): 90-96.