Effects of cyclosporine A and porphyromonas gingivalis-lipopolysaccharide on proliferation of human periodontal ligament fibroblasts in vitro

doi:10.3877/cma.j.issn.1674-1366.2011.05.004

Abstract

Abstract:

Objective

To evaluate the effects of cyclosporine A （CsA） and Porphyromonas gingivalis-lipopolysaccharide（Pg-LPS） on the proliferation of human periodontal ligament fibroblasts（hPDLFs） in vitro.

Methods

hPDLFs were cultured by using modified tissue block culture method. Cells of the 3^rd and 5^th passage were then incubated with CsA （10 ng/ml， 100 ng/mL，200 ng/ml， 400 ng/ml），Pg-LPS（10 ng/ml，100 ng/ml，1000 ng/ml），CsA（100 ng/ml）+Pg-LPS（100 ng/ml） and CsA （100 ng/ml） + Pg-LPS （1000 ng/ml）. Cell proliferation was assessed with MTT assay.

Results

No significant alterations of the cellular morphology could be observed after drug exposure. 100 ng/ml CsA enhanced hPDLFs proliferation；1000 ng/ml Pg-LPS inhibited hPDLFs proliferation， whereas 100 ng/ml CsA significantly decreased such inhibitory effect；hPDLFs proliferation was not significantly affected when incubated with CsA （100 ng/ml） plus Pg-LPS（100 ng/ml）.

Conclusion

At definite concentration，CsA could enhance hPDLFs proliferation，and decrease the inhibitory effect of Pg-LPS of high concentration.

Key words: Cyclosporine A, Porphyromonas gingivalis-lipopolysaccharide, Periodontal ligament fibroblast

Mei-xiang HUANG, Fu-hua YAN, Li-yan YAO, Da-lan LI, Yu-qian ZHENG, Yan-fen LI, Min-kui LIN. Effects of cyclosporine A and porphyromonas gingivalis-lipopolysaccharide on proliferation of human periodontal ligament fibroblasts in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(05): 470-476.

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Figures/Tables 6

References 21

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组别	加药前	加药后第2 天	加药后第3 天	加药后第5 天
CsA 溶剂组	0.0938±0.0111	0.2340±0.0139	0.3436±0.0299	0.6092±0.0309
CsA 10 ng / ml	0.0938±0.0111	0.2358±0.0173^a	0.3398±0.0252^a	0.6148±0.0205^a
CsA 100 ng / ml	0.0938±0.0111	0.2380±0.0208^a	0.3772±0.0228^b	0.6714±0.0295^b
CsA 200 ng / ml	0.0938±0.0111	0.2362±0.0215^a	0.3208±0.0161^a	0.6348±0.0295^a
CsA 400 ng / ml	0.0938±0.0111	0.2198±0.0215^a	0.3274±0.0353^a	0.5632±0.0344^b

组别	加药前	加药后第2 天	加药后第3 天	加药后第5 天
CsA 溶剂组	0.0938±0.0111	0.2340±0.0139	0.3436±0.0299	0.6092±0.0309
CsA 10 ng / ml	0.0938±0.0111	0.2358±0.0173^a	0.3398±0.0252^a	0.6148±0.0205^a
CsA 100 ng / ml	0.0938±0.0111	0.2380±0.0208^a	0.3772±0.0228^b	0.6714±0.0295^b
CsA 200 ng / ml	0.0938±0.0111	0.2362±0.0215^a	0.3208±0.0161^a	0.6348±0.0295^a
CsA 400 ng / ml	0.0938±0.0111	0.2198±0.0215^a	0.3274±0.0353^a	0.5632±0.0344^b

组别	加药前	加药后第2 天	加药后第3 天	加药后第5 天
单纯培养液组	0.0938±0.0111	0.2346±0.0289	0.3352±0.0310	0.6152±0.0274
LPS 10 ng / ml	0.0938±0.0111	0.2338±0.0263^a	0.3428±0.0164^a	0.6052±0.0386^a
LPS 100 ng / ml	0.0938±0.0111	0.2454±0.0331^a	0.3352±0.0226^a	0.5990±0.0113^a
LPS 1000 ng / ml	0.0938±0.0111	0.2112±0.0263^a	0.3002±0.0129^b	0.5846±0.0202^b

组别	加药前	加药后第2 天	加药后第3 天	加药后第5 天
单纯培养液组	0.0938±0.0111	0.2346±0.0289	0.3352±0.0310	0.6152±0.0274
LPS 10 ng / ml	0.0938±0.0111	0.2338±0.0263^a	0.3428±0.0164^a	0.6052±0.0386^a
LPS 100 ng / ml	0.0938±0.0111	0.2454±0.0331^a	0.3352±0.0226^a	0.5990±0.0113^a
LPS 1000 ng / ml	0.0938±0.0111	0.2112±0.0263^a	0.3002±0.0129^b	0.5846±0.0202^b

组别	加药前	加药后第2 天	加药后第3 天	加药后第5 天
单纯培养液组	0.0988±0.0083	0.2380±0.0166	0.3592±0.0321	0.6028±0.0223
CsA 加LPS 100 ng / ml	0.0988±0.0083	0.2310±0.0160^a	0.3630±0.0239^a	0.6106±0.0130^a
CsA 加LPS 1000 ng / ml	0.0988±0.0083	0.2256±0.0124^a	0.3464±0.0158^a	0.5762±0.0133^b

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