Expression and distribution of tissue inhibitors of metalloproteinase 1 in human coronal dentin

doi:10.3877/cma.j.issn.1674-1366.2011.03.007

Abstract

Abstract:

Objective

To investigate the expression and distribution of tissue inhibitors of metalloproteinase 1 （TIMP-1） in human coronal dentin， thereby explore the effect of TIMP-1 on the dentin formation and reconstruction.

Methods

TIMP-1 in coronal dentin from 21 natural teeth was observed by immunohistochemistry method to exhibit its distribution characteristics.The software Image-pro plus 6.0 was applied to calculate the level of immunoreactivity of TIMP-1.

Results

TIMP-1 was detected throughout the human coronal dentin.Immunoreactivities were identified intensely in the odontoblast zone， the pre-dentin zone as well as the zone adjacent to the dentinoenamel junction， however gradually reduced from the inner layer， the middle layer to the outer layer dentin.

Conclusions

TIMP-1 has specific distribution in human coronal dentin，which indicates its possible role in regulating the extracellular matrix formation， reconstruction and mineralization of the dentin.

Key words: Tissue inhibitors of metalloproteinase-1, Matrix metalloproteinases, Dentin, Immunohistochemistry

Na GUO, Yan WANG, Ya HU, Xia-yi WU, Xue-feng LIN. Expression and distribution of tissue inhibitors of metalloproteinase 1 in human coronal dentin[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(03): 274-279.

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Figures/Tables 2

References 16

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