Abstract:
Objective
The purpose of this study was to establish and characterize the model of human tongue squamous cell carcinoma （TSCC） in severe combined immunodeficiency（SCID） mouse.
Methods
Fresh untreated samples of TSCC were implanted subcutaneously in SCID mice.When xenografts reach 10-15 mm in diameter， they were harvested and cutted into small piece and re-implanted into mice.Samples of the original tumor as well as tumor from each passage were prepared for histology evaluation， in situ hybridization and immunohistochemical analysis.
Results
75%（8/12） of the xenografts grew enough to allow passage to subsequent SCID mice， the longest was implant to 5th passage.Histological analysis of H&E section reveal that the xenograft maintain the histology and heterogeneity of the original tumor，Immunohistochemical confirm the human original of the tumor and the host original of the stromal elements.
Conclusion
This model recapitulates many important features of original tumor， it should provide a valuable platform for cancer research.
Key words:
Tongue squamous cell carcinoma,
Xenograft,
Mouse， severe combined immunodeficiency
Hai-kuo TANG, Lei-tao ZHANG, Jin-song HOU, Qian TAO, Hong-zhang HUANG. Primary study on transplantaitionof human tongue squamous cell carcinoma in severe combined immunodeficiency mouse[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(03): 263-267.