Semi-quantitative detection of TNF mRNA in ameloblastoma by RT-PCR

doi:10.3877/cma.j.issn.1674-1366.2009-02-007

Abstract

Abstract:

Objectives

To detect the expression of TNF in ameloblastoma and explore its correlation with ameloblastoma.

Methods

The total RNAs were isolated from tissues of 10 ameloblastoma and 10 paired normal odontogenic cysts respectively.mRNA was purified by oligotex.Both mRNAs from two kinds of tissues were reversely transcribed to cDNAs.mRNA expression of TNF were evaluated by real time quantitative polymerase chain reaction （RT-PCR）and quantified according to an internal control GAPDH.

Results

The relative quantitative expression of TNF mRNA were 1.709±0.655 and 0.873±0.039 respectively.The over expression of TNF was measured in 9 cases of ameloblastoma.

Conclusion

TNF maybe genes closely related to ameloblastoma.

Key words: Ameloblastoma, Odontogenic tumor, Reverse transcriptase polymerase chain reaction, Genes

Li-jian JIANG, Xiao-hua CHEN, Xin-zhong WU, Dao-zhang CAI, Wei-guo LI, Li-juan WANG. Semi-quantitative detection of TNF mRNA in ameloblastoma by RT-PCR[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(02): 159-164.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2009-02-007

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2009/V3/I02/159

Figures/Tables 6

References 8

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基因名称	引物方向	引物序列	引物长度	扩增长度
GAPDH	GAPDH-F	TTCGACAGTCAGCCGCATCTT	21 000	90 000
	GAPDH-R	ATCCGTTGACTCCGACCTTCA	21 000
TNF	TNF-F	GAGAAGATTCGTGGGCTGAT	20 000	169 000
	TNF-R	CCTTGTTCTTGTCCTGTGGG	20 000

基因名称	引物方向	引物序列	引物长度	扩增长度
GAPDH	GAPDH-F	TTCGACAGTCAGCCGCATCTT	21 000	90 000
	GAPDH-R	ATCCGTTGACTCCGACCTTCA	21 000
TNF	TNF-F	GAGAAGATTCGTGGGCTGAT	20 000	169 000
	TNF-R	CCTTGTTCTTGTCCTGTGGG	20 000

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