Abstract:

Objective

The aim of this study is to investigate the effects of cementum attachment protein （CAP） on the proliferation and mineralization of cultured monkey bone marrow stromal cells （BMSCs）in vitro.

Methods

BMSCs were obtained from monkey bone marrow aspiration and cultured in DMEM medium with 10% FBS. The third passage cells were exposed to various concentrations of CAP （0.125， 0.25，0.5， 1 and 2 μg/ml）. Controls were BMSCs cultured in DMEM medium without CAP. BMSCs proliferation rates and alkaline phosphatase （ALP） synthesis were assessed by an MTT assay. Statistical analyses were performed with a parametric one- way analysis of variance （ANOVA） and Stument-Newman-Keuls test for multiple comparisons.

Results

The proliferation rate of BMSCs was significantly up-regulated by CAP at concentrations of 0.5 to 2 μg/ml （P＜0.05） compared with the control group. There was no significant difference between the control group and experimental groups in ALP synthesis.

Conclusion

CAP could promote the proliferating ability of monkey BMSCs in vitro， whereas have little effects on ALP synthesis.

Key words: Cementum attachment protein, Monkey bone marrow stromal cells, Proliferation, Mineralization