Abstract:

The detection of pathogenic microorganisms is a common method for clinical diagnosis of infectious diseases，and its efficient and accurate detection is particularly important in the identification of etiology，diagnosis and treatment.With the continuous development of biotechnology and sensor technology，intelligent and portable biosensors have made great contributions to human health in microbial detection.At present，scholars have discovered an Argonaute protein from Pyrococcus furiosus，which is widely used in the detection of pathogenic microorganisms.Compared with the traditional microbial detection technology，Pyrococcus furiosus Argonaute（PfAgo）enzyme has outstanding advantages and is suitable for rapid and accurate detection of pathogenic microorganisms in various complex environments.This article briefly introduces the development，principle and advantages of PfAgo enzyme，and expounds the application cases of biosensors based on PfAgo enzyme in the detection of pathogenic microorganisms.Comprehensive analysis shows that the biosensor detection technology based on PfAgo enzyme has the advantages of simple operation，low reagent consumption，fast detection speed，low false positive rate and high automation，and has a very broad development space and application prospect for pathogenic microorganism detection.

Key words: PfAgo enzyme, Biosensor, Pathogenic microorganism, Microbial detection