The clinical significance of Th17/Treg cell balance in chronic periodontitis patients with coronary heart disease

doi:10.3877/cma.j.issn.1674-1366.2020.05.005

Abstract

Abstract:

Objective

To investigate the expression levels and clinical significance of peripheral blood helper t cells 17 (Th17) , regulatory t cells (Treg) and Th17/Treg cells in chronic periodontitis patients with coronary heart disease.

Methods

In this study, 2 × 2 factorial design was used. According to outcomes of coronary angiography and the diagnostic criteria of periodontitis, a total of 113 patients with cardiology and stomatology in Xinjiang Uygur Autonomous Region People′s Hospital from March to December 2019 were collected, including 28 healthy control group, 26 chronic periodontitis group, 27 coronary heart disease group and 32 coronary heart disease combined with chronic periodontitis group. The proportion of Th17 and Treg cells in peripheral blood was detected by flow cytometry, and the ratio of Th17/Treg cells was calculated. Variance analysis was used to compare the cell proportion of each group, and 2 × 2 factorial analysis was used to explore the interaction between periodontitis and coronary heart disease on Th17/Treg cell balance.

Results

The imbalance of Th17/Treg was found in the peripheral blood of the patients with coronary heart disease and chronic periodontitis. The proportion of circulating Thl7 cell level was significantly higher in coronary heart disease combined with chronic periodontitis group than in healthy control group, chronic periodontitis group and coronary heart disease group (F = 18.40, P<0.001) . The proportion of circulating Treg cell level was significantly higher in coronary heart disease combined with chronic periodontitis group than in healthy control group (F = 6.29, P = 0.001) .The ratio of Thl7/Treg was significantly higher in coronary heart disease combined with chronic periodontitis group than in healthy control group and chronic periodontitis group (χ² = 40.25, P<0.001) . The factorial analysis demonstrated the effect of periodontitis on Treg cell level in patients with coronary heart disease (F = 3.91, P = 0.041) .

Conclusions

The imbalance of Th17/Treg ratio in patients with periodontitis and coronary heart disease and deviated to Th17 cells indicate that Th17/Treg cell balance may participate in the occurrence and development of periodontitis and coronary heart disease.

Key words: Chronic periodontitits, Coronary disease, Th17 cells, T-lymphocytes, regulatory

Wenjing Xiang, Jianhua He, Beibei Dong, Xiaotao Chen. The clinical significance of Th17/Treg cell balance in chronic periodontitis patients with coronary heart disease[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2020, 14(05): 302-307.

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References 25

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组别	例数	Th17（%, ± s)	Treg（%, ± s)	Th17/Treg[中位数（四分位数间距）]
健康对照组	28	0.51 ± 0.24^abc	8.73 ± 2.70	0.055(0.054)
慢性牙周炎组	26	0.82 ± 0.43^cd	7.18 ± 2.85^d	0.119(0.115)^cd
冠心病组	27	1.02 ± 0.54^cd	5.74 ± 3.04^d	0.185(0.336)^d
牙周炎伴冠心病组	32	1.4 ± 0.6^abd	6.26 ± 2.51^d	0.221(0.317)^ad
检验值	?	F = 18.40	F = 6.29	χ²=40.25
P值	?	<0.001	<0.001	<0.001

组别	例数	Th17（%, ± s)	Treg（%, ± s)	Th17/Treg[中位数（四分位数间距）]
健康对照组	28	0.51 ± 0.24^abc	8.73 ± 2.70	0.055(0.054)
慢性牙周炎组	26	0.82 ± 0.43^cd	7.18 ± 2.85^d	0.119(0.115)^cd
冠心病组	27	1.02 ± 0.54^cd	5.74 ± 3.04^d	0.185(0.336)^d
牙周炎伴冠心病组	32	1.4 ± 0.6^abd	6.26 ± 2.51^d	0.221(0.317)^ad
检验值	?	F = 18.40	F = 6.29	χ²=40.25
P值	?	<0.001	<0.001	<0.001

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