Influence of different titanium surface topographies caused by selective laser melting on the adhesion of oral Streptococcus

doi:10.3877/cma.j.issn.1674-1366.2018.04.002

Abstract

Abstract:

Objective

To investigate the influence of different titanium surface topographies caused by selective laser melting (SLM) on the adhesion of oral Streptococcus.

Methods

Nanonet (NN) and nanotube (NT) topographies were constructed by sandblasting, anodization and alkali treatment on SLM titanium discs. These two groups were compared with sandblasting (SB) and untreated SLM titanium discs. Surface topography, roughness and hydrophilicity were analyzed with a scanning electron microscope, a profilometer and a contact angle measuring device. To evaluate the adhesion of oral Streptococcus on different surface topographies on SLM titanium, Streptococcus mutans (S.mutans) and Streptococcus sanguinis (S.sanguinis) were incubating on the sample surfaces for 24 h. Bacteria colony counting and staining were conducted to check the amount of live and dead bacteria as well as the total amount of bacteria adhered to the surfaces.

Results

The SLM samples showed a microrough waving surface, while SB samples showed microrough waving surface with grooves and ridges. Nanotubes and nanonets were formed on the surfaces of NT and NN samples. The surface roughness of SB, NN and NT samples was lower than that of SLM samples (Ra_SB= 2.87 μm, Ra_NN= 2.90 μm, Ra_NT= 2.65 μm, Ra_SLM= 7.19 μm) , while their hydrophilicity was higher than that of SLM samples (the water contact angles of SLM, SB, NN and NT samples were 76.90°, 64.47°, 23.17°, 44.13°, respectively) . The results of bacteria colony counting showed that NT samples had the lowest bacteria density of both S.mutans (661.29 CFU/mm²) and S.sanguinis (668.45 CFU/mm², P<0.05) . The results of bacteria staining also showed that NT samples had the lowest total mean fluorescence intensity (P<0.05) , which were 661.29 CFU/mm² for S.mutans and 668.45 CFU/mm² for S.sanguinis. The NN samples had the highest proportion of dead bacteria (P<0.05) , which were 0.47 for S.mutans and 0.62 for S.sanguinis.

Conclusions

The anodized nanotubes on microrough SLM substrate exhibited preferable anti-adherence efficacy on oral Streptococcus. The anti-adherence efficacy of alkali treated nanonets on microrough SLM substrate was inferior to anodized nanotubes. However, it possessed certain bactericidal capability.

Key words: Titanium, Topography, medical, Bacteria adhesion, Selective laser melting

Xiucheng Hu, Feilong Deng. Influence of different titanium surface topographies caused by selective laser melting on the adhesion of oral Streptococcus[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2018, 12(04): 205-212.

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Figures/Tables 7

References 22

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组别	例数	Ra（μm）	Rq（μm）	表面水接触角（°）
SLM组	3	7.19 ± 0.33	10.34 ± 0.81	76.90 ± 3.12
SB组	3	2.87 ± 0.10	3.61 ± 0.14	64.47 ± 4.82
NN组	3	2.90 ± 0.14	3.70 ± 0.14	23.17 ± 5.01
NT组	3	2.65 ± 0.06	3.20 ± 0.12	44.13 ± 3.98

组别	例数	Ra（μm）	Rq（μm）	表面水接触角（°）
SLM组	3	7.19 ± 0.33	10.34 ± 0.81	76.90 ± 3.12
SB组	3	2.87 ± 0.10	3.61 ± 0.14	64.47 ± 4.82
NN组	3	2.90 ± 0.14	3.70 ± 0.14	23.17 ± 5.01
NT组	3	2.65 ± 0.06	3.20 ± 0.12	44.13 ± 3.98

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