Effect of c-myc silence on MMPs expression and invasion of immortalized ameloblastoma cell line hTERT+-AM

doi:10.3877/cma.j.issn.1674-1366.2017.05.002

Abstract

Abstract:

Objective

To investigate the effect of c-myc silence on MMPs expression and invasion of immortalized ameloblastoma cell line hTERT⁺-AM.

Methods

Functional characteristics of immortalized ameloblastoma cell line hTERT⁺-AM with stable c-myc silence included migration, invasion, and regulation of matrix metalloproteinases MMP-2, MMP-9 by qRT-PCR and Western blotting. All data were analyzed by SPSS 13.0 software package.

Results

After gene silencing of c-myc in hTERT⁺-AM cells, the mRNA relative expression of c-myc, MMP-2 and MMP-9 was (0.41 ± 0.02) , (0.79 ± 0.05) and (0.76 ± 0.01) respectively, declined by about 60% (LSD-t=-0.591, P<0.001) and 20% (LSD-t_MMP-2=-0.206, LSD-t_MMP-9=-0.242, P<0.001) , the protein expression of c-myc, MMP-2 and MMP-9 was reduced by about 50% (LSD-t_c-myc=-0.461, LSD-t_MMP-2=-0.257, LSD-t_MMP-9=-0.261, P<0.001) , the capacity of migration (±s=24.9 ± 4.5, LSD-t=-17.000, P= 0.021) and invasion (±s=10.8 ± 4.1, LSD-t=-22.250, P= 0.011) was decreased.

Conclusions

Silence of c-myc gene significantly inhibits MMPs expression and cell invasive ability of hTERT⁺-AM cells, suggesting c-myc may be a new target for ameloblastoma treatment.

Key words: Ameloblastoma, C-myc, Matrix metalloproteinase 2, Matrix metalloproteinase 9, Invasion, local

Qixiang Liang, Bin Zhang, Yancan Liang, Hongliang Xie. Effect of c-myc silence on MMPs expression and invasion of immortalized ameloblastoma cell line hTERT⁺-AM[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2017, 11(05): 266-272.

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References 29

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基因	引物序列
GAPDH	F:5′-GCACCGTCAAGGCTGAGAAC-3′
?	R:5′-TGGTGAAGACGCCAGTGGA-3′
c-myc	F:5′-GCAGCTGCTTAGACGCTGGA-3′
?	R:5′-CGCAGTAGAAATACGGCTGCAC-3′
MMP-2	F:5′-CTCATCGCAGATGCCTGGAA-3′
?	R:5′-TTCAGGTAATAGGCACCCTTGAAGA-3′
MMP-9	F:5′-ACGCACGACGTCTTCCAGTA-3′
?	R:5′-CCACCTGGTTCAACTCACTCC-3′

基因	引物序列
GAPDH	F:5′-GCACCGTCAAGGCTGAGAAC-3′
?	R:5′-TGGTGAAGACGCCAGTGGA-3′
c-myc	F:5′-GCAGCTGCTTAGACGCTGGA-3′
?	R:5′-CGCAGTAGAAATACGGCTGCAC-3′
MMP-2	F:5′-CTCATCGCAGATGCCTGGAA-3′
?	R:5′-TTCAGGTAATAGGCACCCTTGAAGA-3′
MMP-9	F:5′-ACGCACGACGTCTTCCAGTA-3′
?	R:5′-CCACCTGGTTCAACTCACTCC-3′

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