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Chinese Journal of Stomatological Research(Electronic Edition) ›› 2016, Vol. 10 ›› Issue (03): 187-192. doi: 10.3877/cma.j.issn.1674-1366.2016.03.007

Special Issue:

• Basic Science Research • Previous Articles     Next Articles

Serum protein adsorption on acid-alkali treated porous titanium

Yujie Wen1, Xuelian Li2, Yitong Yao2, Yutao Jian2,()   

  1. 1. Foshan Stomatological Hospital, Foshan 528000, China
    2. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangdong engineering Research Center of Technology and Materials for Oral Reconstruction, Guangzhou 510055, China
  • Received:2016-04-13 Online:2016-06-01 Published:2016-06-01
  • Contact: Yutao Jian
  • About author:
    Corresponding author: Jian Yutao, Email:

Abstract:

Objective

To evaluate serum protein adsorption on acid-alkali treated porous titanium, thus to provide theoretical evidence for the favourable cytocompatibility.

Methods

Porous titanium discs were fabricated by powder metallurgy process, and then immersed in a mixed acid and NaOH solution to get acid-alkali-treated porous Ti (AAPT) . Fifty-one AAPT discs, 35 non-treated porous Ti (NTPT) discs and 35 non-treated commercial dense titanium (NTDT) discs were made. The surface morphology of NTDT was observed under a scanning electron microscopy (SEM) . Contact angle and specific surface area (SSA) of three groups were observed and studied by means of an optical contact angle measuring device and a specific surface area and pore size analyser. Protein adhesion of different time was quantitatively analyzed by BCA method. The level of significance was determined by One-Way ANOVA followed by a least significant difference (LSD) t-test for a multiple comparison procedure.

Results

NTDT presented smooth surface under SEM. The contact angle on the surface of AAPT (22.08°) and NTDT (75.69°) exhibited a statistically significantly lower in comparison to the contact angle of NTPT (93.7°) (F= 392.02, P<0.001) . SSA of AAPT (27.05) was higher than NTDT (3.74) and NTPT (18.36) (F= 4586.10, P<0.001) . At different period, the protein adsorption of AAPT was higher than that of NTDT and NTPT (P<0.001) .

Conclusion

The amount of serum protein adsorption could be significantly improved on acid-alkali treated porous titanium.

Key words: Titanium, Porosity, Surfactant, Protein adsorption

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