Effect of platelet-derived growth factors and fluid shear stress on cell proliferation and c-fos expression in osteoblasts

doi:10.3877/cma.j.issn.1674-1366.2015.02.006

Abstract

Abstract:

Objectives

To study the effect of platelet-derived growth factor (PDGF) and fluid shear stress (FSS) on cell proliferation and c-fos expression in osteoblasts.

Methods

Different concerntrations of PDGF (0, 10, 30, 50 ng/ml) were used to stimulate primary cultured osteoblasts, respectively. CCK-8 and real-time quantitative PCR were performed to detect cell proliferation and c-fos mRNA expression. Furthermore, osteoblasts were stimulated by PDGF and loaded by FSS of 12 dyne/cm² for 1 h, together. CCK-8, real-time quantitative PCR and western blot were performed to detect cell proliferation, c-fos mRNA and protein expression, respectively.

Results

PDGF or FSS can respectively increase cell proliferation (F_FSS = 6.500, P_FSS< 0.05; F_PDGF = 6.077, P_PDGF< 0.05) and the expression levels of c-fos mRNA in osteoblasts (F_FSS = 6.425, P_FSS< 0.05; F_PDGF = 7.549, P_PDGF< 0.05) . There was no synergistic effect between PDGF and FSS (F_{proliferation} = 1.826, P_{proliferation}> 0.05; F_{c-fos mRNA} = 2.101, P_{c-fos mRNA}> 0.05; F_{c-fos protein} = 1.561, P_{c-fos protein}> 0.05) .

Conclusion

PDGF itself or combined with FSS can increase cell proliferation and c-fos expression levels in osteoblasts, but there is no synergistic effect between PDGF and FSS on cell proliferation and c-fos expression in osteoblasts.

Key words: Platelet-derived growth factor, Fluid shear stress, Osteoblasts, c-fos, Proliferation

Xiaodan Chen, Yinghui Xiang, Feng Qin, Zhi Yang, Shuyi Tan, Zijun Song, Qiang Fu. Effect of platelet-derived growth factors and fluid shear stress on cell proliferation and c-fos expression in osteoblasts[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(02): 118-122.

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Figures/Tables 9

References 20

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基因	引物序列
c-fos	上游：5′-TTACGCCAGAGCGGGAATG-3′
c-fos	下游：5′-GTTCCCTTCGGATTCTCCGTTT-3′
GAPDH	上游：5′-GGTCATCCCAGAGCTGAACG-3′
GAPDH	下游：5′-TTGCTGTTGAAGTCGCAGGA-3′

基因	引物序列
c-fos	上游：5′-TTACGCCAGAGCGGGAATG-3′
c-fos	下游：5′-GTTCCCTTCGGATTCTCCGTTT-3′
GAPDH	上游：5′-GGTCATCCCAGAGCTGAACG-3′
GAPDH	下游：5′-TTGCTGTTGAAGTCGCAGGA-3′

处理因素	离均差平方和	自由度	均方	F值	P值
FSS	0.084	1	0.084	6.500	0.034^a
PDGF	0.079	1	0.079	6.077	0.039^a
FSS + PDGF	0.024	1	0.024	1.826	0.214

处理因素	离均差平方和	自由度	均方	F值	P值
FSS	0.084	1	0.084	6.500	0.034^a
PDGF	0.079	1	0.079	6.077	0.039^a
FSS + PDGF	0.024	1	0.024	1.826	0.214

处理因素	离均差平方和	自由度	均方	F值	P值
FSS	0.165	1	0.165	6.425	0.035^a
PDGF	0.194	1	0.194	7.549	0.025^a
FSS + PDGF	0.054	1	0.054	2.101	0.185

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