Beclin1-targeted microRNA-30a inhibits autophagic activity in tongue squamous cell carcinoma cell lines

doi:10.3877/cma.j.issn.1674-1366.2015.02.002

Abstract

Abstract:

Objective

To investigate the effect of microRNA (miRNA) -30a on the expression of beclin1 and the autophagic activity in tongue squamous cell carcinoma cell lines.

Methods

MiRNA-30a mimic and inhibitor were transfected into tongue squamous cell line cell UM1, UM2 and SCC25 with the lipofection transfection. Cells were cultured and divided into 3 groups which including miRNA-30a mimic group, miRNA-30a inhibitor group and control group. The expression of Beclin1 and LC3 was analyzed using real-time quantitative polymerase chain reaction and western blotting.

Results

Transfection with miRNA-30a mimic caused a decrease in the mRNA and protein expression of Beclin1 compared with control group (P< 0.05) . Meanwhile, the expression of LC3-Ⅱ protein was significantly lower than the control group (P< 0.05) ; By contrast, treatment with the miRNA-30a inhibitor resulted in an increase in beclin1 mRNA and protein (P< 0.05) . The LC3-Ⅱprotein was significantly increased as well in this group (P< 0.05) .

Conclusion

MiRNA-30a plays a suppressive effect on the expression of Beclin1 and autophagic activity in tongue squamous cell carcinoma cell lines.

Key words: MicroRNA-30a, Beclin1, Tongue, Carcinoma, squamous cell, Autophagy

Jianfeng Liang, Cheng Wang, Junquan Weng, Guanhui Chen, Jinsong Hou. Beclin1-targeted microRNA-30a inhibits autophagic activity in tongue squamous cell carcinoma cell lines[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(02): 95-99.

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https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2015/V09/I02/95

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