Study the role of rat bone marrow mesenchymal stem cells on temporomandibular joint disk cells

doi:10.3877/cma.j.issn.1674-1366.2014.05.004

Abstract

Abstract:

Objective

To study the role of rat bone marrow mesenchymal stem cells on disc cells from TMJ.

Methods

Culture rat bone marrow mesenchymal stem cells. These cells were induced into osteoblasts， chondrocytes， adipocytes to prove the potential of multilineage differentiation. The ColⅡof rat disc cells immunofluorescence assay in immunofluorescence microscopic. Prepared bone msenchyme stem cells conditioned medium （BMSC-CM） to culture rat TMJ disc cells. Cell Counting Kit-8 （CCK-8）analysis cell proliferation in conditioned medium. RT-PCR evaluated the changes in transcription genes of Col Ⅰ， Col Ⅱ， Col Ⅹ at 7 and 14 d.

Results

Rat BMSCs were successfully induced into osteoblasts， chondrocytes， adipocytes. ColⅡimmunofluorescence were detected in immunofluorescence microscopic. CCK-8 showed after culture 48 h， the disc cells proliferation increased condition medium compared with control group. RT-PCR revealed that Col Ⅰ， Col Ⅱ， Col Ⅹwere different between conditioned medium group and control group.

Conclusion

Rat BMSCs promote the disc cells proliferation and decrease the ColⅩexpression protect the disc cells.

Key words: Rats, Condition medium, Temporomandibular joint disk, Bone mesenchymal stem cell

Chao Xing, Yangpeng Sun, Yuliang Zheng, Wenjing Liu, Youhua Zheng, Zhiguang Zhang. Study the role of rat bone marrow mesenchymal stem cells on temporomandibular joint disk cells[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(05): 370-375.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2014.05.004

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2014/V8/I05/370

Figures/Tables 5

References 28

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目的基因	序列5'-3'	产物长度（bp）	基因库登记号
ColⅠ	F：5'-CTACAGCACGCTTGTGGATG-3'	195	Z_78279
	R：5'-CAGATTGGGATGGAGGGAGT-3'
ColⅡ	F：5'-AGAACTGGTGGAGCAGCAAGA-3'	124	NM_012929
	R：5'-ATCTGGACGTTAGCGGTGTTG-3'
ColⅩ	F：5'-CCATGGTTCACACAACCCCTT-3'	129	AJ131848.1
	R：5'-TGGCTGTGGTAAAGCACCTTG-3'
GAPDH	F：5'-GGCACAGTCAAGGCTGAGAATG-3'	143	M_0170084
	R：5'-ATGGTGGTGAAGACGCCAGTA-3'

目的基因	序列5'-3'	产物长度（bp）	基因库登记号
ColⅠ	F：5'-CTACAGCACGCTTGTGGATG-3'	195	Z_78279
	R：5'-CAGATTGGGATGGAGGGAGT-3'
ColⅡ	F：5'-AGAACTGGTGGAGCAGCAAGA-3'	124	NM_012929
	R：5'-ATCTGGACGTTAGCGGTGTTG-3'
ColⅩ	F：5'-CCATGGTTCACACAACCCCTT-3'	129	AJ131848.1
	R：5'-TGGCTGTGGTAAAGCACCTTG-3'
GAPDH	F：5'-GGCACAGTCAAGGCTGAGAATG-3'	143	M_0170084
	R：5'-ATGGTGGTGAAGACGCCAGTA-3'

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