Effect of orthodontic force on the expression of C-C chemokine receptor 1 and its associated ligands mRNA in periodontium of rats

doi:10.3877/cma.j.issn.1674-1366.2014.03.006

Abstract

Abstract:

Objective

This study was to explore the role of C-C chemokine receptors 1（CCR1） in orthodontic tooth movement by studying the change of the mRNA expression of CCR1 and its related ligands （CCL3， CCL5， CCL7） in the periodontium of rat molars after orthodontic force loading.

Methods

Thirty-five male Sprague-Dawley rats， 8 weeks old and （220 ± 25） g in weight，were randomly divided into 7 groups， 5 rats in a group. Experimental devices were not placed to blank control group rats，but were placed to the remaining rats. Randomly select one side of the maxillary first molar of the experimental group to load an orthodontic force of 50 gram with a Nickel-Titanium extension spring， and the contralateral first molars were used as control. The rats were euthanized after 0 h， 12 h， 1 d， 3 d， 7 d， 14 d of mechanical loading， and the periodontal tissue surrounding maxillary first molars were collected for analysis of real-time quantitative PCR.

Results

The mRNA expression of CCR1 and its related ligands showed a certain regularity over time after mechanical loading. Following experimental tooth movement， the mRNA expression of CCR1， CCL3， CCL5， CCL7 were increased after 12 h. The mRNA expression of CCR1 reached a peak after mechanical loading by 3 d （F=1.745， P=0.021）， while CCL3， CCL5 by 1 d （F=19.976， P_CCL3=0.019； F=17.114， P_CCL5=0.008）. There was no statistical difference in the mRNA expression of CCL7. The mRNA expression of CCR1 and its related ligands were decreased consistent with the control group after 7d of mechanical loading.

Conclusions

The mRNA expression of CCR1 and its related ligands in periodontium of rats had a short-term increase after mechanical loading. These results suggested that the expression of CCR1 and its related ligands were probably related with orthodontic tooth movement and periodontal bone remodeling.

Key words: Orthodontic force, Sprague-Dawley rats, Periodontium, C-C chemokine receptor 1, Real-time quantitative PCR

Yixi Cai, Chufeng Liu, Yang Cao, Wenjia Lai, Lingchao Zhang. Effect of orthodontic force on the expression of C-C chemokine receptor 1 and its associated ligands mRNA in periodontium of rats[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2014, 8(03): 208-214.

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Figures/Tables 5

References 18

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基因	序列
CCR1	上游：5'-GAACTGGAGCAGAGAGGACAGA-3'
	下游：5'-GAAGCATCACAGTGCTCACCA-3'
CCL3	上游：5'-GAGCCCCAGAACATTCCTGC-3'
	下游：5'-GGATGTGGCTACTTGGCAGC-3'
CCL5	上游：5'-CTGCATCCCTCACCGTCATC-3'
	下游：5'-GTGTCCGAGCCATATGGGGA-3'
CCL7	上游：5'-GCCCTGGAGACCTCATGTCA-3'
	下游：5'-AGGAACAAAGTCAATGGGGCG-3'
β-actin	上游：5'-CATTGTCACCAACTGGGACGATA-3'
	下游：5'-GGATGGCTACGTACATGGCTG-3'

基因	序列
CCR1	上游：5'-GAACTGGAGCAGAGAGGACAGA-3'
	下游：5'-GAAGCATCACAGTGCTCACCA-3'
CCL3	上游：5'-GAGCCCCAGAACATTCCTGC-3'
	下游：5'-GGATGTGGCTACTTGGCAGC-3'
CCL5	上游：5'-CTGCATCCCTCACCGTCATC-3'
	下游：5'-GTGTCCGAGCCATATGGGGA-3'
CCL7	上游：5'-GCCCTGGAGACCTCATGTCA-3'
	下游：5'-AGGAACAAAGTCAATGGGGCG-3'
β-actin	上游：5'-CATTGTCACCAACTGGGACGATA-3'
	下游：5'-GGATGGCTACGTACATGGCTG-3'

时间	目的基因	实验组	对照组	P值（实验组与对照组）	P值（对照组与空白组）
0 h	CCR1	1.067±0.150	0.972±0.248	0.490	0.778
	CCL3	1.095±0.146	0.939±0.217	0.226	0.579
	CCL5	1.103±0.303	1.115±0.287	0.951	0.547
	CCL7	1.197±0.340	1.220±0.379	0.922	0.240
12 h	CCR1	1.245±0.334	1.249±0.392	0.988	0.271
	CCL3	12.929±3.554	1.295±0.414	0.000^a	0.209
	CCL5	2.007±0.590	1.520±0.287	0.136	0.055
	CCL7	1.292±0.279	1.314±0.512	0.936	0.245
1 d	CCR1	1.416±0.371	1.213±0.208	0.317	0.146
	CCL3	14.225±6.044	1.264±0.454	0.001^a	0.273
	CCL5	5.304±1.754	1.714±0.494	0.008^a	0.061
	CCL7	1.490±0.363	1.331±0.346	0.499	0.100
3 d	CCR1	1.581±0.330	1.063±0.166	0.021^b	0.659
	CCL3	10.337±5.166	1.552±0.543	0.019^b	0.089
	CCL5	2.497±0.558	1.354±0.404	0.007^a	0.134
	CCL7	1.326±0.301	1.152±0.362	0.433	0.387
7 d	CCR1	1.323±0.158	1.134±0.387	0.724	0.543
	CCL3	2.609±1.265	1.473±0.756	0.132	0.246
	CCL5	1.906±0.339	1.666±0.504	0.407	0.072
	CCL7	1.107±0.308	1.124±0.245	0.926	0.333
14 d	CCR1	1.332±0.443	1.269±0.240	0.791	0.098
	CCL3	1.622±0.565	1.446±0.434	0.536	0.088
	CCL5	1.070±0.200	1.195±0.285	0.451	0.293
	CCL7	1.125±0.213	1.195±0.374	0.728	0.315

时间	目的基因	实验组	对照组	P值（实验组与对照组）	P值（对照组与空白组）
0 h	CCR1	1.067±0.150	0.972±0.248	0.490	0.778
	CCL3	1.095±0.146	0.939±0.217	0.226	0.579
	CCL5	1.103±0.303	1.115±0.287	0.951	0.547
	CCL7	1.197±0.340	1.220±0.379	0.922	0.240
12 h	CCR1	1.245±0.334	1.249±0.392	0.988	0.271
	CCL3	12.929±3.554	1.295±0.414	0.000^a	0.209
	CCL5	2.007±0.590	1.520±0.287	0.136	0.055
	CCL7	1.292±0.279	1.314±0.512	0.936	0.245
1 d	CCR1	1.416±0.371	1.213±0.208	0.317	0.146
	CCL3	14.225±6.044	1.264±0.454	0.001^a	0.273
	CCL5	5.304±1.754	1.714±0.494	0.008^a	0.061
	CCL7	1.490±0.363	1.331±0.346	0.499	0.100
3 d	CCR1	1.581±0.330	1.063±0.166	0.021^b	0.659
	CCL3	10.337±5.166	1.552±0.543	0.019^b	0.089
	CCL5	2.497±0.558	1.354±0.404	0.007^a	0.134
	CCL7	1.326±0.301	1.152±0.362	0.433	0.387
7 d	CCR1	1.323±0.158	1.134±0.387	0.724	0.543
	CCL3	2.609±1.265	1.473±0.756	0.132	0.246
	CCL5	1.906±0.339	1.666±0.504	0.407	0.072
	CCL7	1.107±0.308	1.124±0.245	0.926	0.333
14 d	CCR1	1.332±0.443	1.269±0.240	0.791	0.098
	CCL3	1.622±0.565	1.446±0.434	0.536	0.088
	CCL5	1.070±0.200	1.195±0.285	0.451	0.293
	CCL7	1.125±0.213	1.195±0.374	0.728	0.315

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