Expression of nucleotide-binding oligomerization domain 2 in inflamed human dental pulp tissues

doi:10.3877/cma.j.issn.1674-1366.2013.04.002

Abstract

Abstract:

Objective

To investigate the expression and distribution of nucleotide-binding domain leucine-rich repeat containing receptor nucleotide-binding oligomerization domain 2 （NOD2） in inflamed human dental pulp tissues， and explore the possible role of NOD2 in dental pulp innate immunity.

Methods

The protein and messenger RNA level of NOD2 in healthy and inflamed human dental pulp tissues were detected by western blotting and fluorescence quantitative-polymerase chain reaction （qPCR）. The expression of NOD2 in the pulp tissues were detected by immunohistochemistry.

Results

The NOD2 mRNA level was significantly up-regulated in inflamed dental pulp tissues compared to healthy ones； NOD2 protein were detected in both healthy and inflamed dental pulp tissues by Western blot，while the expression level was increased in inflamed ones. Immunohistochemistry results demonstrated that NOD2 is strongly positive stained in inflammatory cells and pulp fibroblasts among inflamed dental pulp tissues， while it was not obvious in pulp fibroblasts in healthy pulp tissues.

Conclusions

These findings suggest that NOD2 participates in the dental pulp inflammatory and immunity response，indicating that NOD2 might play an important role in the immune defense of human dental pulp.

Key words: Dental pulp, Innate immunity, Inflammation, Nucleotide-binding oligomerization domain 2

Lan JIANG, Zhi SONG, Run-fu WANG, Shu-heng HUANG, Zheng-mei LIN. Expression of nucleotide-binding oligomerization domain 2 in inflamed human dental pulp tissues[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2013, 07(04): 265-270.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2013.04.002

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2013/V07/I04/265

Figures/Tables 4

References 22

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基因	引物序列	大小（bp）
GAPDH	F: 5'-GACTCATGACCACAGTCCATG-3'	79
	R: 5'-CCATCACGCCACAGTTTC-3'
	Probe: 5'-（FAM）CATCACTGCCACCCAGAAGACT-（Tamra）3'
NOD2	F:5'-CAGGATGAAGGTGTATGTTCTCTC-3'	224
	R:5'-CTTCAAAGCAAGAGTCTGGTGTC-3'
	Probe: 5'-（FAM）ACTTGAATTTTTCTTCAGTCCTTCTGC-（Tamra）3'

基因	引物序列	大小（bp）
GAPDH	F: 5'-GACTCATGACCACAGTCCATG-3'	79
	R: 5'-CCATCACGCCACAGTTTC-3'
	Probe: 5'-（FAM）CATCACTGCCACCCAGAAGACT-（Tamra）3'
NOD2	F:5'-CAGGATGAAGGTGTATGTTCTCTC-3'	224
	R:5'-CTTCAAAGCAAGAGTCTGGTGTC-3'
	Probe: 5'-（FAM）ACTTGAATTTTTCTTCAGTCCTTCTGC-（Tamra）3'

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