Analysis of relationship between symptoms of the clinical patients and the detection of enterococcus faecalis in extraradicular biofilm of refractory periapical periodontitis

doi:10.3877/cma.j.issn.1674-1366.2012.06.007

Abstract

Abstract:

Objective

Identification and detection of Enterococcus faecalis （E.faecalis） isolated from extraradicular biofilm in refractory periapical periodontitis， in order to analyze the relationship between the occurrence of E.faecalis and clinical symptom.

Methods

Forty-two single root teeth which need periapical surgery were collected， and the clinical symptoms and physical signs were recorded.Bacterium samples from extraradicular biofilm were taken. The occurrence of E.faecalis by means of culture and polymerase chain reaction was investigated. The presence of E.faecalis and clinical signs was assessed to investigate the potential relationship between clinical findings.

Results

Culture and PCR revealed that the detection rates of E.faecalis was 52.4% and 71.4%， respectively， there was statistical significance （P ＜0.05）； The detection rates of E.faecalis by means of PCR between the pain and the painless group showed statistical significance （P ＜0.05）.

Conclusions

PCR was sensitive in detecting E.faecalis in root apex of refractory periapical periodontitis. The occurrence of E.faecalis in cases of refractory periapical periodontitis correlates with pain.

Key words: Refractory periapical periodontitis, Biofilm, Enterococcus faecalis

Lei MENG, Zhi-shan LU, Zhong-hao LIU, Tao-wen ZHANG, Guang-hui REN. Analysis of relationship between symptoms of the clinical patients and the detection of enterococcus faecalis in extraradicular biofilm of refractory periapical periodontitis[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 06(06): 512-515.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2012.06.007

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2012/V06/I06/512

Figures/Tables 4

References 9

1	Wada M， Takase T， Nakanuma K， et al. Clinical study of refractory apical periodontitis treated by apicectomy. Part 1.Root canal morphology of resected apex. Int Endod J， 1998，31（1）：53-56.
2	Signoretti FG， Endo MS， Gomes BP， et al. Persistent extraradicular infection in root-filled asymptomatic human tooth：scanning electron microscopic analysis and microbial investigation after apical microsurgery. J Endod， 2011，37（12）：1696-1700.
3	Nair PN. On the causes of persistent apical periodontitis：a review. Int Endod J， 2006，39（4）：249-281.
4	Noguchi N， Noiri Y， Narimatsu M， et al. Identification and localization of extraradicular biofilm-forming bacteria associated with refractory endodontic pathogens. Appl Environ Microbiol，2005，71（12）：8738-8743.
5	Ricucci D， Siqueira JF Jr. Biofilms and apical periodontitis：study of prevalence and association with clinical and histopathologic findings. J Endod， 2010，36（8）：1277-1288.
6	Su L， Gao Y， Yu C， et al. Surgical endodontic treatment of refractory periapical periodontitis with extraradicular biofilm.Oral Surg Oral Med Oral Pathol Oral Radiol Endod， 2010，110（1）：e40-e44.
7	Vidana R， Sullivan A， Billström H， et al. Enterococcus faecalis infection in root canals-host-derived or exogenous source？ Lett Appl Microbiol， 2011，52（2）：109-115.
8	Gomes BP， Pinheiro ET， Sousa EL， et al. Enterococcus faecalis in dental root canals detected by culture and by polymerase chain reaction analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod， 2006，102（2）：247-253.
9	Cogulu D， Uzel A， Oncag O， et al. Detection of Enterococcus faecalis in Necrotic Teeth Root Canals by Culture and Polymerase Chain Reaction Methods. Eur J Dent， 2007，1（4）：216-221.

组别	病例数	检出例数	检出率（%）	χ ²	P
疼痛组	30	25	83.3	7.29	＜0.05
窦道组	11	7	63.6	0.07	＞0.05
无症状组	8	4	50.0	1.12	＞0.05

组别	病例数	检出例数	检出率（%）	χ ²	P
疼痛组	30	25	83.3	7.29	＜0.05
窦道组	11	7	63.6	0.07	＞0.05
无症状组	8	4	50.0	1.12	＞0.05

[1]	Youhan Yang, Yinxian Wen, Liaobin Chen. Research progress of bacteriophage in treatment of periprosthetic joint infection [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2024, 18(01): 125-130.
[2]	Ruipeng Sun, Jing Sun, Liankui Zhao, Dongjun Li, Qiao Huai, Lijuan Xu. Clinical therapeutic effect of modified chitin bioremediation film in treating superficial partial-thickness burn [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2019, 14(04): 280-284.
[3]	Li Zhang, Yang Zhang, Jingjing Ma, Zhehao Yu, Liang Ge, Linchun Sun. Surface-anchored protein SasX involves in the biofilm formation and pathogenicity of Staphylococcus aureus ST239 clone by regulating RNAⅢ [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(04): 252-259.
[4]	Fei Lin, Mingyong Yuan, Baodong Ling. Biofilm formation related genes of Acinetobacter baumannii [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(02): 129-132.
[5]	Fenghua Luo, Jianlin Wei. Relationship between the biofilm formation ability of Acinetobacter baumannii and biofilm related genes, drug resistance [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2021, 15(01): 67-71.
[6]	Minghao Lu, Hong Li, Benxiang Hou. Research progress of periapical biofilm removal methods [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2024, 18(04): 257-261.
[7]	Yanqing Zhang, Shaoxia Feng, Yu Chen. The effect of Lactococcus lactis on the growth and biofilm formation of cariogenic bacteria [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2024, 18(02): 96-102.
[8]	Liping Yao, Lei Zhang, Yuhong Lin, Menghan Zhang, Zhishan Lu. Effect of nanosilver solution used as intracanal medication on dog′s teeth with chronic apical periodontitis [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2020, 14(02): 95-100.
[9]	Haozhen Ma, Xiaolan Li. The applications and perspectives of organotypic oral mucosal infection model in host-pathogen interactions study [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(06): 321-327.
[10]	Xiaolan Li, Xiao Wang, Junqi Ling, Xiaoli Hu, Dongmei Deng. Application of green fluorescent protein reporter system in Streptococcus mutans for study on dual-species biofilms [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2019, 13(03): 136-143.
[11]	Zhong Li. Fecal Enterococcus faecalis for prediction of recurrence in colorectal cancer [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2021, 10(01): 81-85.
[12]	Hang Dong, Donghao Lang, Xue Fu, Fang Li, Lingfeng Wang. Research status of quorum sensing system and mechanism of biofilm resistance [J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(01): 57-60.
[13]	Qianjun Li, Shuhong Huang, Yiyi Lei, Yonglin Feng. Effect of Coptis chinensis on ALS gene expression of Fluconazole resistant Candida albicans in biofilm state [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(02): 89-94.
[14]	Xinglian Mai, Siyao Liu, Xuan Chen, Qianjun He, Zhongwen Zhang, Guoquan Zhong, Weihong Wen, Lingqing Xu. Correlational study between drug resistance and resistance genes of Enterococcus faecalis in urinary tract infection [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2021, 09(02): 65-70.
[15]	Ruifu Yang, Yanbin Zhou. Research progress on the correlation of major facilitator superfamily membrane transporters and bacterial biofilm formation [J]. Chinese Journal of Diagnostics(Electronic Edition), 2023, 11(02): 77-81.

Please choose a citation manager

Content to export