Experiment study on the role and mechanism of TGF-β1/ERK1/2/MMP-2 signaling pathways in the migration and invasion process of ACC

doi:10.3877/cma.j.issn.1674-1366.2012.01.005

Abstract

Abstract:

Objective

To investigate the role of TGF-β1/ERK1/2/MMP-2 signaling pathways in the migration and invasion process of salivary adenoid cystic carcinoma cells.

Methods

ACC-2 cell line were used in this study. The proliferation of ACC-2 cell line was observed by MTT assay； cell migration and invasion ablity were examined by Transwell； the expression of MMP-2 in salivary adenoid cystic carcinoma cell lines was tested by Western blotting and Real-time PCR. The activity of ERK1/2 signaling pathway was measured by Western blotting. The effects of the ERK pathway specific inhibitors on TGF-β1-activated MMP-2 expression in ACC-2 cell lines were examined.

Results

TGF-β1 and U0126 could not enhance the proliferation of ACC-2 cells； TGF-β1 could enhance the migration and invasion abilities of them significantly. TGF-β1 enhanced expression of p-ERK1/2 and MMP-2 protein and MMP-2 mRNA in ACC-2 cells. When U0126 inhibited ERK1/2 pathway， the abilities of migration and invasion and production of MMP-2 stimulated by TGF-β1 were significantly inhibited in ACC-2 cells.

Conclusions

TGF-β1/ERK1/2/MMP-2 signaling pathways participate in the regulation of migration and invasion abilities in ACC. TGF-β1 can up-regulate ERK1/2， and then enhanced MMP-2 expression， promote the migration and invasion abilities of ACC. ERK1/2 may be turned to be a new target spot to prevent the invasion of ACC.

Key words: Adenoid cystic carcinoma, Transforming growth factor-β1, Extracellular regulated protein kinases, Matrix metalloproteinase-2, Signal path

Hong-liang XIE, Bin ZHANG, Zhi-ying XU, Qi-Xiang LIANG. Experiment study on the role and mechanism of TGF-β1/ERK1/2/MMP-2 signaling pathways in the migration and invasion process of ACC[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2012, 6(01): 29-37.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2012.01.005

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Figures/Tables 9

References 18

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组别	细胞增殖率（%）	P
对照组	（100.00±0.00）
TGF-β1（10 ng/ml）	（103.54±2.50）^a	1.000
TGF-β1（10 ng/ml）+U0126（10 μmol/L）	（100.22±4.91）^a	1.000
TGF-β1（10 ng/ml）+U0126（25 μmol/L）	（102.53±5.35）^a	1.000
TGF-β1（10 ng/ml）+U0126（50 μmol/L）	（102.68±4.64）^a	1.000

组别	细胞增殖率（%）	P
对照组	（100.00±0.00）
TGF-β1（10 ng/ml）	（103.54±2.50）^a	1.000
TGF-β1（10 ng/ml）+U0126（10 μmol/L）	（100.22±4.91）^a	1.000
TGF-β1（10 ng/ml）+U0126（25 μmol/L）	（102.53±5.35）^a	1.000
TGF-β1（10 ng/ml）+U0126（50 μmol/L）	（102.68±4.64）^a	1.000

组别	p-ERK1/2	P	MMP2	P
T0	T10	0.018^a	T10	0.000^a
	T10+U10	1.000	T10+U10	0.462
	T10+U25	1.000	T10+U25	0.000^a
	T10+U50	0.010^a	T10+U50	0.000^a
T10	T10+U10	0.059	T10+U10	0.000^b
	T10+U25	0.022	T10+U25	0.000^b
	T10+U50	0.000^b	T10+U50	0.000^b

组别	p-ERK1/2	P	MMP2	P
T0	T10	0.018^a	T10	0.000^a
	T10+U10	1.000	T10+U10	0.462
	T10+U25	1.000	T10+U25	0.000^a
	T10+U50	0.010^a	T10+U50	0.000^a
T10	T10+U10	0.059	T10+U10	0.000^b
	T10+U25	0.022	T10+U25	0.000^b
	T10+U50	0.000^b	T10+U50	0.000^b

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