Expression of HIF-1α and its relationship with local angiogenesis in constant distraction osteogenesis in mandibles of dogs

doi:10.3877/cma.j.issn.1674-1366.2008.05.007

Abstract

Abstract:

Objective

To investigate the expression of HIF-1α in mandibular distraction osteogenesis using TiNi-SMA distractor and to determine the possible effects of HIF-1α on angiogenesis of distracted side.

Methods

Twelve Beagle dogs were enrolled. A 1.5 cm×1.0 cm bone defect was created in both sides of mandible in ten dogs and same shape transport bone disc was created mesially by corticotomy. TiNi-SMA distractor was settled to perform bone transport to repair defect. Two dogs were sacrified on 1， 4， 7， 10 and 15 days after operation seperatively and specimen were harvested. Two dogs were used as control without bone distraction but corticotomy was underwent in same shape in mandible and sacrified 10 days after operation. RT-PCR was employed to investigate the temporal mRNA expression levels of HIF-1α. Immunohistochemical analysis was used to verified the expression of HIF-1α and VEGF proteins. Microvessel density was also determined by detecting CD34 expression. The results were analysed by SPSS 11.5 statistical software.

Results

Positive expression of HIF-1α mRNA were detected 4，7，10 and 15 days after operation in distracted area by RT-PCR and immunohistochemical analysis respectively. Both was expressed mainly in active osteoblasts and peaked on 7th day postoperatively. VEGF expressed positively 4，7，10 and 15 days after operation and expressed both in active osteoblasts and vascular endothelial cells in distracted area， with an increase from 4th day to 10th days after operation （peaked on day 10）. The values of microvessel density also increased during distraction period and peaked on the 7th day postoperatively （P ＜0.05）. There were no apparent expression of HIF-1α mRNA， HIF-1α protein and VEGF in control group.

Conclusion

HIF-1α was highly expressed in the period of constant mandibular distraction osteogenesis. It plays an important role in the end period of distraction and may accelerate angiogenesis in distracted side by upregulating the expression of VEGF.

Key words: Distraction osteogenesis, HIF-1α, Angiogenesis, TiNi-SMA

Peng ZHANG, Rong-sheng ZENG, Cheng WANG. Expression of HIF-1α and its relationship with local angiogenesis in constant distraction osteogenesis in mandibles of dogs[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(05): 467-475.

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Figures/Tables 15

References 19

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	术后1 d	术后4 d	术后7 d	术后10 d	术后15 d	对照组
间充质细胞	-	+	+	+	+/-	-
成骨细胞	+/-	++	+++	++	+	-
内皮细胞	-	-	-	-	-	-

	术后1 d	术后4 d	术后7 d	术后10 d	术后15 d	对照组
间充质细胞	-	+	+	+	+/-	-
成骨细胞	+/-	++	+++	++	+	-
内皮细胞	-	-	-	-	-	-

	术后1 d	术后4 d	术后7 d	术后10 d	术后15 d	对照组
间充质细胞	-	-	+	+	+/-	-
成骨细胞	+/-	+	+++	+++	++	+/-
内皮细胞	-	-	+	++	++	-

	术后1 d	术后4 d	术后7 d	术后10 d	术后15 d	对照组
间充质细胞	-	-	+	+	+/-	-
成骨细胞	+/-	+	+++	+++	++	+/-
内皮细胞	-	-	+	++	++	-

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