A study on the expression of CXCR4 in tongue carcinoma and metastatic cervical lymph node

doi:10.3877/cma.j.issn.1674-1366.2008.04.003

Abstract

Abstract:

Objective

To detect the mRNA and protein level of CXCR4 in human tongue carcinoma and metastatic cervical lymph nodes.

Methods

The mRNA of CXCR4 in the human tongue carcinoma and metastatic cervical lymph node from 41 paient was tested by semiquantitative RT-PCR. Strept Avidin-Biotin-Complex Method（SABC Method） was employed to delect the localization， intensity and distribution of CXCR4 protein.

Results

Immunohistochemistry staining and RT-PCR demonstrated that CXCR4 was expressed in tongue carcinoma and metastatic cervical lymph nodes， and was not or just weakly expressed in normal tounge tissue and lymph nodes.

Conclusions

The mRNA and protein of CXCR4 tongue carcinoma and metastatic cervical lymph node. The expressions of CXCR4 is correlated with the prognosis and cervical lymph node metastasis of tongue carcinoma.

Key words: CXC chemokine receptor 4, Tongue carcinoma, Metastasis

Shuang-xi ZHU, Jun-bing GUO, Hong-wei JIANG, Dai-jun KUANG. A study on the expression of CXCR4 in tongue carcinoma and metastatic cervical lymph node[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(04): 329-335.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2008.04.003

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2008/V02/I04/329

Figures/Tables 6

References 14

1	Müller A， Homey B， Soto H， et al. Involvement of chemokine receptors in breast cancer metastasis. Nature， 2001，410（6824）：50-56.
2	Horuk R. Chemokine receptors. Cytokine Growth Factor Rev， 2001，12（4）：313-335.
3	Woo SU， Bae JW， Kim CH， et al. A significant correlation between nuclear CXCR4 expression and axillary lymph node metastasis in hormonal receptor negative breast cancer. Ann Surg Oncol， 2008，15（1）：281-285.
4	Wehler T， Wolfert F， Schimanski CC， et al. Strong expression of chemokine receptor CXCR4 by pancreatic cancer correlates with advanced disease. Oncol Rep， 2006，16（6）：1159-1164.
5	Ou DL， Chen CL， Lin SB， et al. Chemokine receptor expression profiles in nasopharyngeal carcinoma and their association with metastasis and radiotherapy. J Pathol， 2006，210（3）：363-373.
6	Kijima T， Maulik G， Ma PC， et al. Regulation of cellular proliferation， cytoskeletal function， and signal transduction through CXCR4 and c-Kit in small cell lung cancer cells. Cancer Res， 2002，62（21）：6304-6311.
7	Libura J， Drukala J， Majka M， et al. CXCR4-SDF-1 signaling is active in rhabdomyosarcoma cells and regulates locomotion， chemotaxis，and adhesion. Blood， 2002，100（7）：2597-2606.
8	Vlahakis SR， Villasis-Keever A， Gomez T， et al. G protein-coupled chemokine receptors induce both survival and apoptotic signaling pathways. J Immunol， 2002，169（10）：5546-5554.
9	Prasad A， Fernandis AZ， Rao Y， et al. Slit protein-mediated inhibition of CXCR4-induced chemotactic and chemoinvasive signaling pathways in breast cancer cells. J Biol Chem， 2004，279（10）：9115-9124.
10	Liotta LA. An attractive force in metastasis. Nature， 2001，410（6824）：24-25.
11	Su LP， Zhang JP， Xu HB， et al.The role of CXCR4 in lung cancer metastasis and its possible mechanism. Zhonghua Yi Xue Za Zhi，2005，85（17）：1190-1194.
12	Kucia M， Reca R， Miekus K， et al. Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms：pivotal role of the SDF-1-CXCR4 axis. Stem Cells， 2005，23（7）：879-894.
13	Uchida D， Begum NM， Almofti A， et al. Possible role of stromal-cell-derived factor-1/CXCR4 signaling on lymph node metastasis of oral squamous cell carcinoma. Exp Cell Res， 2003，290（2）：289-302.
14	Uchida D， Begum NM， Tomizuka Y， et al. Acquisition of lymph node， but not distant metastatic potentials， by the overexpression of CXCR4 in human oral squamous cell carcinoma. Lab Invest， 2004，84（12）：1538-1546.

临床病理参数	例数	CXCR4				P值
临床病理参数	例数	-	+	++	+++	P值
性别
男	23	9	5	3	6
女	18	4	5	5	4	＞0.05
年龄（岁）
＜50	22	3	7	7	5
≥50	19	10	3	1	5	＞0.05
T分期
T1-T2	29	11	9	5	4
T3-T4	12	2	1	3	6	＜0.05
分化程度
高分化	22	6	4	6	6
中-低分化	19	7	6	2	4	＞0.05
淋巴结转移
N0	25	11	6	5	3
N+	16	2	4	3	7	＜0.05
临床分期
Ⅰ-Ⅱ	20	9	6	3	2
Ⅲ-Ⅳ	21	4	4	5	8	＜0.05

临床病理参数	例数	CXCR4				P值
临床病理参数	例数	-	+	++	+++	P值
性别
男	23	9	5	3	6
女	18	4	5	5	4	＞0.05
年龄（岁）
＜50	22	3	7	7	5
≥50	19	10	3	1	5	＞0.05
T分期
T1-T2	29	11	9	5	4
T3-T4	12	2	1	3	6	＜0.05
分化程度
高分化	22	6	4	6	6
中-低分化	19	7	6	2	4	＞0.05
淋巴结转移
N0	25	11	6	5	3
N+	16	2	4	3	7	＜0.05
临床分期
Ⅰ-Ⅱ	20	9	6	3	2
Ⅲ-Ⅳ	21	4	4	5	8	＜0.05

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