Effect of β-tricalcium phosphate ceramic on the biological behavior of periosteal cells from Beagle dogs

doi:10.3877/cma.j.issn.1674-1366.2008.03.008

Abstract

Abstract:

Objective

To evaluate the effect of β-TCP on proliferation and differentiation of periosteal cells.

Methods

Periosteal cells isolated from Beagle dogs were cultured by tissue culture method in vitro. Cells were transferred in culture， and inoculated onto the surface of β-TCP. The proliferation and differentiation of periosteal cells were measured through MTT， flow cytometry （FCM） and alkaline phosphate （ALP） kit， and cellular morphology was examined by scanning electronic microscope.

Results

Periosteal cells could adhere to and proliferate on the surface of β-TCP. A higher alkaline phosphatase activity was also shown.

Conclusion

β-TCP has shown good biocompatibility and may be considered as an acceptable scaffold for periodontal tissue engineering.

Key words: Periosteal, β-tricalcium phosphate, Proliferation, Differentiation, Tissue engineering

Xiao-hong WU, Fu-hua YAN, Min-kui LIN, Yan-fen LI, Xin ZHAO. Effect of β-tricalcium phosphate ceramic on the biological behavior of periosteal cells from Beagle dogs[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(03): 249-254.

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Figures/Tables 8

References 9

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组别	2 d	4 d	6 d	7 d
β-TCP组	0.082±0.002	0.201±0.003^*	0.281±0.008	0.286±0.004
对照组	0.083±0.003	0.187±0.007	0.292±0.012	0.295±0.009

组别	2 d	4 d	6 d	7 d
β-TCP组	0.082±0.002	0.201±0.003^*	0.281±0.008	0.286±0.004
对照组	0.083±0.003	0.187±0.007	0.292±0.012	0.295±0.009

组别	G₀/G₁	S	G₂/M
β-TCP组	88.90±0.46	7.04±0.64	4.06±0.84
对照组	87.47±1.42	7.76±0.43	4.77±1.58

组别	G₀/G₁	S	G₂/M
β-TCP组	88.90±0.46	7.04±0.64	4.06±0.84
对照组	87.47±1.42	7.76±0.43	4.77±1.58

组别	5 d	8 d	14 d
β-TCP组	0.418±0.010	0.810±0.034	0.980±0.024
对照组	0.430±0.009	0.832±0.017	1.001±0.008

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