The humoral immune response to oral candidiasis in two strains of inbred mice

doi:10.3877/cma.j.issn.1674-1366.2008.03.002

Abstract

Abstract:

Objective

The aims of this study were to compare the oral infection with C.albicans in two inbred mice which are genetically susceptible or resistant to candidiasis using a murine model， and to explore the patterns of antibody production from these two mice after oral challenge with C.albicans.

Methods

Inbred female BALB/c and CBA/CaH mice and C.albicans 3630 were used in this study. Oral infection was established on murine mouth by inoculation of live C.albicans， and monitored by swabbing. The antibody production profile of mouse was determined with Western Blotting after oral immunization with yeasts.

Results

CBA/CaH mice demonstrated a severer oral infection and a stronger humoral response the infection by producing IgG1， IgG2a and IgM than BALB/c mice.

Conclusions

Oral infection pattern in this study is consistent with the pattern of systemic infection for these two strains of inbred mice， confirming that the genetic factors play a key role for severity of both oral and systemic candidiasis.However， C.albicans-specific IgM might play a protective role for oral Candida infection in mice.

Key words: Inbred mice, Oral candidiasis, Humoral immunity

Xiao-huan ZHANG, Yan HU, S Farah Camile, B Ashman Robert. The humoral immune response to oral candidiasis in two strains of inbred mice[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(03): 211-216.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2008.03.002

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2008/V02/I03/211

Figures/Tables 3

References 17

1	Fidel PL， Jr. Immunity to Candida. Oral Dis， 2002，8（Suppl 2）：69-75.
2	Snydman DR. Shifting patterns in the epidemiology of nosocomial Candida infections. Chest， 2003，123（5 Suppl）：500-503.
3	Farah CS， Elaihi S， Drysdal K， et al. Primary role for CD4 （+） T lymphocytes in recovery from oropharyngeal candidiasis. Infect Immun，2002，70（2）：724-731.
4	Ashman RB， Farah CS， Hu Y， et al. Genetic models of Candida infection and host resistance factors. Drug Discovery Today： Disease Models， 2005，2（2）：155-159.
5	Farah CS， Hu Y， Riminton S， et al. Distinct roles for IL-12p40 and TNF in resistance to oral candidiasis defined by gene-targeting. Oral Microbiol Immunol， 2006，21（4）：252-255.
6	Costantino PJ， Gare NF， Warmington JR. Humoral immune responses to systemic Candidia albicans infection in inbred mouse strains.Immunol Cell Biol， 1995，73（2）：125-133.
7	Pitarch A， Diez-Orejas R， Molero G， et al. Analysis of the serologic response to systemic Candida albicans infection in a murine model.Proteomics， 2001，1（4）：550-559.
8	Hu Y， Farah CS， Ashman RB. Isolates of Candida albicans that differ in virulence for mice elicit strain-specific antibody-mediated protective responses. Microbes Infect， 2006，8（3）：612-620.
9	Papadimitriou JM， Ashman RB. The pathogenesis of acute systemic candidiasis in a susceptible inbred mouse strain. J Pathol， 1986，150（4）：257-265.
10	Chakir J. Differential pattern of infection and immune response during experimental oral candidiasis in BALB/c and DBA/2 （H-2d） mice.Oral Microbiol Immunol， 1994，9（2）：88-94.
11	Farah CS， Elahi S， Pang G， et al. T cells augment monocyte and neutrophil function in host resistance against oropharyngeal candidiasis.Infect Immun， 2001，69（10）：6110-6118.
12	Farah CS， Gotjamanos T， Seymour GJ， et al. Cytokines in the oral mucosa of mice infected with Candida albicans. Oral Microbiol Immunol， 2002，17（6）：375-378.
13	Ashman RB， Papadimitriou JM. Production and function of cytokines in natural and acquired immunity to Candida albicans infection.Microbiol Rev， 1995，59（4）：646-672.
14	Ashman RB. Candida albicans： pathogenesis， immunity and host defence. Res Immunol， 1998，149（4-5）：281-288.
15	Han YM， Morrison RP， Cutler JE. A vaccine and monoclonal antibodies that enhance mouse resistance to Candida albicans vaginal infection. Infect Immun， 1998，66（12）：5771-5776.
16	Montagnoli C. Immunogenicity and protective effect of recombinant enolase of Candida albicans in a murine model of systemic candidiasis.Med Mycol， 2004，42（4）：319-324.
17	Matthews R. Candida and AIDS： evidence for protective antibody. Lancet， 1988，2（8605）：263-266.

[1]	Xiuxia Wu, Yuxia Chen, Qianqian Fan. Systematic review and clinical significance of immune function in neonates with infectious pneumonia [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(02): 128-133.
[2]	Zhibin Yang, Enrui Shen, Li Pan, Lihui Zhao, Cong Wang, Yanxia Yang, Yang Li, Qiaofeng Wang, Shiwu Ma. Expression and clinical significance of serum interleukin-21 in patients with different types of tuberculosis [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2019, 13(01): 48-53.
[3]	Li Ren, Mingna Huang, Jiling Feng, Xihua Wu, Shiya Su. Study on the changes of cellular immunity and humoral immunity in patients with cerebral infarction [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2018, 08(06): 343-347.
[4]	Jun Liu. Immunological monitoring in critically ill associated immune dysfunction [J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2019, 05(01): 56-63.
[5]	Mingjing Wang, Yonggang Xu, Xiaoqing Ding, Richeng Quan, Haiyan Chen, Zhongyang Deng, Pan Zhao, Xiaomei Hu. Cellular immune function in patients with immune thrombocytopenia [J]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(02): 65-69.

Please choose a citation manager

Content to export