Fabrication of sustained release rbFGF/PLGA implanted tablets and release study in vitro

doi:10.3877/cma.j.issn.1674-1366.2008-02-005

Abstract

Abstract:

Objective

To fabricate sustained release basic fibroblast growth factor/poly lactide-co-glycolide （rbFGF/PLGA） implanted tablets and observe their general properties，drug-release characteristics in vitro， and the biologic activity of rbFGF in vivo.

Methods

The rbFGF/PLGA implanted tablets were fabricate by solvent evaporation method. rbFGF content was determined by double-antibody sandwich ELISA test， drug-release rate and content was detected by during drug-release test in vitro. The tablets was implanted into the rabbit model of mandibular distraction osteogenesis to investigate the biologic activity of rbFGF.

Results

The surface of rbFGF/PLGA implanted tablets have the feature of smoothness， integrity and uniformity， with a sustained release role in vitro. The content of rbFGF is 3.613 μg per tablet. The equation for the standard curve of the tablet is C=1280.54A-99.21 （n = 7， r²=0.9952）， with a linear range between 15.6- 1000 pg/ml. The in vitro rbFGF release rates of the tablet were 10.70%， 51% and above 80% in the 1st, 9th and 20th day respecfively. In the rabbit model of mandibular distraction osteogenesis in which the rbFGF/PLGA tablets were implanted， the formation rate of new bone and the density of bone trabeculae were superior to those of the control group.

Conclusions

The rbFGF/PLGA implanted tablets have excellent pharmaceutical properties, showing sustained release effect in vitro with good biological activity in vivo. Bone formation and maturation was accelerated during mandibular distraction osteogenesis in rabbits.

Key words: Basic fibroblast growth factor, Poly lactide-co-glycolide, Distraction osteogenesis

Ming WANG, Xiao-ping YANG, yu-xiong SU, Xi YANG, Xiao-yan HU. Fabrication of sustained release rbFGF/PLGA implanted tablets and release study in vitro[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2008, 02(02): 130-134.

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URL: https://zhkqyxyjzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-1366.2008-02-005

https://zhkqyxyjzz.cma-cmc.com.cn/EN/Y2008/V02/I02/130

Figures/Tables 4

References 7

1	田建广，白东海，刘志国，等. 聚乳酸-羟基乙酸胶原膜生物相容性的实验研究. 中华烧伤杂志， 2003，11（Z1）：1-4.
2	Pean JM， Menei P， Montero-Menei CN， et al. Intraseptal implantation of NGF-releasing microspheres promote the survival of axotomized cholinergic neurons. Biomaterials， 2000， 21（20）：2097-2101.
3	Alpaslan C， Irie K， Takahashi K， et al. Long-term evaluation of recombinant human bone morphogenetic protein-2 induced bone formation with a biologic and synthetic delivery system. Br J Oral Maxillofac Surg， 1996，34（5）：414-418.
4	Eppley BL， Sadove AM， Havlik RJ. Resorbable plate fixation in Rediatric craniofacial surgery. Plast Reconstr Surg， 1997， 100（1）：1-7.
5	Bergsma JE， Fred R， Rozema J， et al. Foreign body reactions of resorbable poly （L-lactide）/bone plates and screws used for the fixation of unstable zygomatic fractures. J Oral Maxillofac Surg， 1993，51（6）：666-670.
6	Thompson JA， Anderson KD， Dipietro JM， et al. Site-directed neovessel formation in vivo. Science， 1988，241（4871）：1349-1352.
7	Edelman ER， Mathiowitz E， Langer R， et al. Controlled and modulated release of basic fibroblast growth factor. Biomaterials， 1991，12（7）：619-626.

时间（d）	累积释药量（μg）	累积释药百分数（%）	时间（d）	累积释药量（μg）	累积释药百分数（%）
1	0.39±0.12	10.7±1.2	11	2.10±0.28	58.2±5.4
3	0.71±0.24	19.7±2.4	14	2.43±0.22	67.2±6.3
5	0.79±0.14	22.0±3.4	17	2.77±0.35	76.7±7.8
7	1.32±0.26	36.5±5.6	20	2.98±0.32	82.7±7.2
9	1.84±0.21	51.0±4.8

时间（d）	累积释药量（μg）	累积释药百分数（%）	时间（d）	累积释药量（μg）	累积释药百分数（%）
1	0.39±0.12	10.7±1.2	11	2.10±0.28	58.2±5.4
3	0.71±0.24	19.7±2.4	14	2.43±0.22	67.2±6.3
5	0.79±0.14	22.0±3.4	17	2.77±0.35	76.7±7.8
7	1.32±0.26	36.5±5.6	20	2.98±0.32	82.7±7.2
9	1.84±0.21	51.0±4.8

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