Abstract:

Objective

To evaluate the effects of transplanting mesenchymal stem cells（MSCs） and natural coral （NC） complex combined with guided tissue regeneration （GTR） on the treatment of periodontal bone defects in the Mongrel dogs.

Methods

（1）MSCs isolated from the Mongrel dogs were cultured with mineralization medium for osteogenic differentiation. The osteogenic ability of cells was examined. （2）MSCs were seeded into porous NC particles and PLGA-NC complex membrane， the morphological characteristics of MSCs were observed with phase contrast microscope and scanning electron microscope. （3）After 10 days of co-culture of MSCs and NC， the complexes were transplanted into experimentally induced alveolar bone defects of dogs by GTR therapy using PLGA-NC membranes. The defects were histologically assessed 8 weeks later.

Results

（1）MSCs after induction showed osteogenic potential. The staining of mineralized nodules and type Ⅰcollagen were both positive. （2）Good biocompatibility was observed for MSCs and NC， as well as MSCs and the PLGA-NC membrane. （3）The defects were restored by transplanting with MSCs-NC complex.

Conclusions

MSCs was the potential precursor cells for periodontal tissue regeneration. It is indicated that bone tissue-engineering could be applied to restore periodontal defects.

Key words: Mesenchymal stem cells, Guided tissue regeneration, Natural coral, Biocompatibility