Abstract:
Objective
To isolate and identify Nanobacteria from dental calculus.
Methods
Freshly collected 15 dental calculus were used for the isolation and culture of Nanobacteria. The morphology and growth characteristics of the cultured bacteria were examined. Nanobacteria was identified by von Kossa stain， immunohistochemical and immunofluorescence stain with 8D10 and G1B8 antibodies， as well as double stain including Hoechst stain and von Kossa stain.
Results
The morphology and growth characteristics of the bacteria isolated from 15 dental calculus were similar to that of Nanobacteria. Von Kossa stain， immunohistochemical stain， and immunofluorescence stain with nanobacteria-specific monoclonal antibodies were positive. In double staining test， Hoechst staining was negative while von Kossa staining was positive. In contrast， Hoechst staining of the dental pulp cells was positive.
Conclusion
The bacteria isolated from dental calculus in this study has been shown to be Nanobacteria. The unique biological characteristics of Nanobacteria may play an important role in the formation of dental calculus.
Key words:
Dental calculus,
Nanobacteria,
Extraskeletal calcification
Junqi LING, Jin-feng ZENG, Hong-wei JIANG, Bang-peng REN. Isolation，cultivation and initial identification of Nanobacteria from dental calculus[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2007, 01(01): 3-6.