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中华口腔医学研究杂志(电子版) ›› 2015, Vol. 09 ›› Issue (06) : 449 -454. doi: 10.3877/cma.j.issn.1674-1366.2015.06.003

所属专题： 文献；

基础研究

聚甲基丙烯酸β-羟乙酯对牙本质基质金属蛋白酶活性的影响
周晶1, 罗有成1, 孙素1, 王冠林2, 苏军1, 肖玉鸿1,†()   
  1. 1. 650032　昆明，成都军区昆明总医院口腔科，昆明医科大学教学医院
    2. 650540　昆明理工大学生命与技术学院
  • 收稿日期:2015-10-13 出版日期:2015-12-01
  • 通信作者: 肖玉鸿
  • 基金资助:
    国家自然科学基金(81460107)

The effect of poly (2-hydroxyethyl methacrylate) on the activity of matrix metalloproteinases in dentin

Jing Zhou1, Youcheng Luo1, Su Sun1, Guanlin Wang2, Jun Su1, Yuhong Xiao1,†()   

  1. 1. Department of Stomatology, Kunming General Hospital of Chengdu Military Command, Teaching Hospital of Kunming Medical University, Kunming 650032, China
    2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650504, China
  • Received:2015-10-13 Published:2015-12-01
  • Corresponding author: Yuhong Xiao
  • About author:
    Corresponding author: Xiao Yuhong, Email: , Tel: 0871-64774939
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引用本文：

周晶, 罗有成, 孙素, 王冠林, 苏军, 肖玉鸿. 聚甲基丙烯酸β-羟乙酯对牙本质基质金属蛋白酶活性的影响[J]. 中华口腔医学研究杂志(电子版), 2015, 09(06): 449-454.

Jing Zhou, Youcheng Luo, Su Sun, Guanlin Wang, Jun Su, Yuhong Xiao. The effect of poly (2-hydroxyethyl methacrylate) on the activity of matrix metalloproteinases in dentin[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(06): 449-454.

目的

研究聚甲基丙烯酸β-羟乙酯（PHEMA）对牙本质中基质金属蛋白酶（MMP）的影响。

方法

通过本体聚合将树脂单体甲基丙烯酸β-羟乙酯（HEMA）制备成PHEMA，同时制备人牙本质粉，并随机平均分成6组，对照组不做处理，实验组以不同高度的PHEMA试件处理，提取牙本质蛋白，用Ⅰ型胶原吡啶交联终肽（ICTP）试剂盒分析样本中ICTP的浓度，应用SPSS 17.0软件包对实验数据进行统计学分析，采用单因素方差（One-Way ANOVA）分别分析PHEMA和时间这两个处理因素对的ICTP影响，选择LSD-t检验进行组间两两比较，明胶酶谱法检测PHEMA对牙本质中MMP-2、MMP-8、MMP-9活性的影响，通过Image J图像分析软件，采用灰度法进行图像分析。

结果

ICTP浓度与MMP活性之间存在正相关关系。PHEMA可有效抑制ICTP的浓度（F = 26.792，P = 0.001），且ICTP浓度随着时间延长而降低。PHEMA可有效抑制MMP-2（F = 15.317，P = 0.0009）、MMP-8（F = 6.475，P = 0.004）的活性，且抑制作用随接触面积的增大而增强；PHEMA对MMP-9（F = 1.093，P = 0.413）未出现明显的活性抑制作用。

结论

PHEMA可有效抑制牙本质中MMP-2、MMP-8的活性，且抑制作用随接触面积的增大而增强。

关键词: 甲基丙烯酸β-羟乙酯, 基质金属蛋白酶类, 牙本质, Ⅰ型胶原吡啶交联终肽
Objective

The purpose of this study was to test the effect of poly (2-hydroxyethyl methacrylate) on the matrix metalloproteinase in dentin.

Methods

PHEMA was prepared by the synthesis of methyl methacrylate by Bulk polymerization. Meanwhile, human dentin powder was prepared and divided into 6 groups as follows: The group without the treatment of PHEMA was used for control samples, and the other experimental groups were treated with different heights of PHEMA. Extracting the dentin protein from the six groups, the concentration of ICTP in the sample was analyzed by Cross-linked carboxyterminaltelopeptide typeⅠ collagen reagent box. The software of SPSS 17.0 was used for statistical analysis. ANOVA One-Way were used to analyze the effects of PHEMA and time on the ICTP, and LSD-t test for comparison between two groups. As well as the activity of MMP-2, 8, 9 in dentin was detection by Zymography, Using the gray method for image analysis through the J Image analysis software.

Results

There was a positive correlation between the concentration of ICTP and the activity of MMPs. PHEMA can inhibit the concentration of ICTP (F = 26.792, P = 0.001) and the effect of inhibition was decreased by increasing time. PHEMA can inhibit the activity of MMP-2 (F = 15.317, P = 0.0009) and MMP-8 (F = 6.475, P = 0.004) , the effect of inhibition was increased by increasing the contact area; the activity of MMP-9 was not significantly inhibited by PHEMA.

Conclusion

PHEMA can effectively inhibit the activity of MMP-2, 8 of the dentin, and the inhibition increased with the increase of contact area.

Key words: Hydroxyethyl Methacrylates, Matrix metalloproteinases, Dentin, Cross-linked carboxyterminaltelopeptide of typeⅠ collagen
图1 不同规格的聚甲基丙烯酸β-羟乙酯试件
图2 不同时间相同接触面积PHEMA对牙本质中ICTP浓度的影响
图3 相同时间不同接触面积PHEMA对牙本质中ICTP浓度的影响
图4 不同接触面积PHEMA对牙本质中MMP-2、MMP-8、MMP-9的抑制情况
图5 不同接触表面积PHEMA与灰度值间线性回归分析
图6 ICTP浓度与MMP浓度的线性回归分析
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