Effects of advanced glycation end products on the proliferation and MMP-1 synthesis of human gingival fibroblast

doi:10.3877 / cma.j.issn.1674-1366.2009-03-003

Abstract

Abstract:

Objective

To investigate the effects of advanced glycation end products（AGEs）on the proliferation and matrix matelloproteinase-1 （MMP-1） synthesis of human gingival fibroblast （HGF）.

Methods

AGEs were isolated in vitro by means of both tissue explant cultivation and enzyme digestion methods. Different concentrations of AGEs were added into the culture media. At different time points， MTT colorimetric assay and ELISA were used to measure the changes of the proliferation rate and MMP-1 levels of HGF， respectively.

Results

AGEs inhibited HGF proliferation in a dose-dependent manner （P＜0.05）. Compared to the control group， MMP-1 synthesis was increased significantly in a dose-dependent manner （P＜0.05）.

Conclusions

The AGEs may inhibit the proliferation of HGF while promote MMP-1 synthesis，and the latter may increase collagen degradation. Thereby， AGEs may accelerate periodontal destruction process in diabetes.

Key words: Human gingival fibroblast （HGF）, Advanced glycation end products（AGEs）, Proliferation, Matrix matelloproteinast-1 （MMP-1）

Yu-quan DENG, Yun FU, Liu-ying QI, Zhi-ying TANG. Effects of advanced glycation end products on the proliferation and MMP-1 synthesis of human gingival fibroblast[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(03): 250-254.

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Figures/Tables 5

References 13

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AGE-HSA（μg /ml）	1 d	2 d	3 d
50	17.25±0.52^*	7.41±4.17^*	4.86±1.33^*
100	33.83±3.92^*▲	25.92±2.46^*▲	14.24±4.81^*▲
200	40.82±5.13^*▲■	96.72±2.39^*▲■	93.64±3.12^*▲■

AGE-HSA（μg /ml）	1 d	2 d	3 d
50	17.25±0.52^*	7.41±4.17^*	4.86±1.33^*
100	33.83±3.92^*▲	25.92±2.46^*▲	14.24±4.81^*▲
200	40.82±5.13^*▲■	96.72±2.39^*▲■	93.64±3.12^*▲■

AGE-HSA（μg/ml）	MMP-1的浓度
0	26.29±2.53
0.5	48.71±3.85^*
5	51.09±2.37^*▲
50	63.11±4.45^*▲■
100	80.22±9.56^*▲■★

AGE-HSA（μg/ml）	MMP-1的浓度
0	26.29±2.53
0.5	48.71±3.85^*
5	51.09±2.37^*▲
50	63.11±4.45^*▲■
100	80.22±9.56^*▲■★

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