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中华口腔医学研究杂志(电子版) ›› 2017, Vol. 11 ›› Issue (03) : 142 -148. doi: 10.3877/cma.j.issn.1674-1366.2017.03.003

所属专题: 文献

基础研究

Toll样受体4抑制剂对糖尿病小鼠体内肿瘤坏死因子α及精氨酸酶1表达的影响
韩倩倩1, 戚威娟1, 刘钊2, 杨熙1,()   
  1. 1. 510280 广州,南方医科大学口腔医院·广东省口腔医院牙周科
    2. 510515 广州,南方医科大学南方医院牙体牙髓病科
  • 收稿日期:2017-01-13 出版日期:2017-06-01
  • 通信作者: 杨熙
  • 基金资助:
    国家自然科学基金(青年科学基金项目,81500848); 广东省医学科研基金(A2015196、A2015145)

The effects of TAK-242 on the expression of TNF-α and Arg1 in diabetic mouse

Qianqian Han1, Weijuan Qi1, Zhao Liu2, Xi Yang1,()   

  1. 1. Department of Periodontology, Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China
    2. Department of Endodontics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
  • Received:2017-01-13 Published:2017-06-01
  • Corresponding author: Xi Yang
  • About author:
    Corresponding author: Yang Xi, Email:
引用本文:

韩倩倩, 戚威娟, 刘钊, 杨熙. Toll样受体4抑制剂对糖尿病小鼠体内肿瘤坏死因子α及精氨酸酶1表达的影响[J/OL]. 中华口腔医学研究杂志(电子版), 2017, 11(03): 142-148.

Qianqian Han, Weijuan Qi, Zhao Liu, Xi Yang. The effects of TAK-242 on the expression of TNF-α and Arg1 in diabetic mouse[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2017, 11(03): 142-148.

目的

探讨Toll样受体4(TLR4)抑制剂TAK-242对糖尿病及牙周炎小鼠血清中肿瘤坏死因子α(TNF-α)表达及牙龈组织中TNF-α及精氨酸酶1(Arg1)表达的影响。

方法

建立糖尿病、牙周炎及糖尿病复合牙周炎小鼠模型,并给予TAK-242注射,10 d后取血清运用Elisa方法检测不同小鼠血清中TNF-α的表达;同时获取不同处理组小鼠的牙龈组织,实时荧光定量聚合酶链反应(PCR)检测小鼠牙龈组织中TNF-α及Arg1的表达,数据分析采用独立样本t检验及单因素方差分析。

结果

糖尿病组、牙周炎组及糖尿病复合牙周炎组小鼠血清中TNF-α及牙龈组织中TNF-α表达较对照组升高,TAK-242的应用可降低血清中TNF-α及牙龈组织中TNF-α表达(F血清=25.329,P血清<0.001;F牙龈=47.901,P牙龈<0.001);而糖尿病组、牙周炎组及糖尿病复合牙周炎组小鼠牙龈组织中Arg1的表达降低,应用TAK-242后Arg1表达上调(F= 45.897,P<0.001)。

结论

TAK-242可调控炎症相关因子TNF-α及Arg1的表达,可能在牙周炎、糖尿病以及糖尿病复合牙周炎的慢性炎症过程中发挥重要作用。

Objective

To investigate the effect of TLR4 inhibitor TAK-242 on the expression of TNF-α in serum and TNF-α and Arg1 mRNA expression in periodontal tissues of diabetic mice and periodontitis mice.

Methods

Groups of diabetes, periodontitis and periodontitis combined with diabetes mouse models were successfully established and TAK-242 was applied in different groups separately. After 10 days, Elisa method was used to detect the expression of TNF-α in mouse serum and real time PCR was used to detect the mRNA expression of TNF-α and Arg1 in gingival tissue. One-Way ANOVA was applied to analyze the data.

Results

The expression of TNF-α in mice serum and gingival tissue was increased within diabetic group, periodontitis group and diabetic periodontitis group. The application of TAK-242 could reduce the expression level of TNF-α (Fserum= 25.329, Pserum<0.001; Fgingival tissue= 47.901, Pgingival tissue<0.001) . The expression of Arg1 in diabetes mellitus, periodontitis group and diabetic periodontitis group decreased in gingival tissues while upregulated after treated with TAK-242 (F= 45.897, P<0.001) .

Conclusions

TAK-242 could regulate the expression of TNF-α and Arg1 and might play an important role in the chronic inflammation of periodontitis, diabetes and diabetic periodontitis.

表1 实验基因引物序列
图1 糖尿病小鼠与健康对照组相比葡萄糖曲线下面积的差异
图2 小鼠牙周炎病变模型建立
图3 不同处理组血液中TNF-α的表达
图4 不同处理组牙龈中TNF-α mRNA的表达
图5 不同处理组牙龈中Arg1 mRNA的表达
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