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中华口腔医学研究杂志(电子版)

中华口腔医学研究杂志(电子版) ›› 2016, Vol. 10 ›› Issue (03) : 161 -165. doi: 10.3877/cma.j.issn.1674-1366.2016.03.002

所属专题： 文献；

基础研究

舌鳞状细胞癌上皮-间质转化与血管生成拟态的关系及意义
陈纯1, 赵小朋2, 吴宇翎2, 黎润东1, 潘朝斌2,†()   
  1. 1. 511300　广州市增城区人民医院（中山大学孙逸仙纪念医院增城院区）口腔科
    2. 510120　广州，中山大学孙逸仙纪念医院口腔颌面外科
  • 收稿日期:2015-11-11 出版日期:2016-06-01
  • 通信作者: 潘朝斌
  • 基金资助:
    广东省科技计划(2012B031800252); 教育部高等学校博士学科点专项科研基金(20130171110095)

Significance of epithelial-mesenchymal transition and its relation with vasculogenic mimicry in human tongue squamous cell carcinoma

Chun Chen1, Xiaopeng Zhao2, Yuling Wu2, Rundong Li1, Chaobin Pan2,†()   

  1. 1. Department of Stomatology, Zengcheng District People′s Hospital of Guangzhou City, Guangzhou 511300, China
    2. Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Received:2015-11-11 Published:2016-06-01
  • Corresponding author: Chaobin Pan
  • About author:
    Corresponding author: Pan Chaobin, Email:
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引用本文：

陈纯, 赵小朋, 吴宇翎, 黎润东, 潘朝斌. 舌鳞状细胞癌上皮-间质转化与血管生成拟态的关系及意义[J]. 中华口腔医学研究杂志(电子版), 2016, 10(03): 161-165.

Chun Chen, Xiaopeng Zhao, Yuling Wu, Rundong Li, Chaobin Pan. Significance of epithelial-mesenchymal transition and its relation with vasculogenic mimicry in human tongue squamous cell carcinoma[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2016, 10(03): 161-165.

目的

初步探讨舌鳞状细胞癌（TSCC）上皮-间质转化（EMT）与血管生成拟态（VM）的关系及意义。

方法

对65例TSCC组织及相应癌旁正常组织采用免疫组化检测EMT标记物E-cadherin和Vimentin的表达情况，同时CD34联合PAS双重染色检测VM情况。

结果

Vimentin在癌和癌旁正常上皮的阳性表达率分别是41.54%和0%，差异具有统计学意义（Z=-5.815，P= 0.000），Vimentin表达与肿瘤组织分化、临床分期及有无淋巴结转移密切相关。E-cadherin在TSCC和癌旁正常上皮的阳性表达率分别是阳性率56.92%和100%，差异具有统计学意义（Z=-5.951，P= 0.000），E-cadherin表达与临床分期及有无淋巴结转移密切相关。VM在TSCC和癌旁正常组织的出现的例数比率是33.14%和0%，差异具有统计学意义（Z=-3.946，P= 0.000），VM与肿瘤临床分期及有无淋巴结转移密切相关。相关分析显示，E-cadherin与Vimentin具有负相关关系（r=-0.507，P= 0.000），Vimentin表达与VM具有正相关关系（r= 0.592，P= 0.000），VM与E-cadherin具有负相关关系（r=-0.518，P= 0.000）。

结论

TSCC中存在EMT及VM，存在EMT和VM的TSCC具有更高临床分期和容易发生淋巴结转移，两者具有密切相关性，对TSCC侵袭、转移具有重要作用。

关键词: 舌, 癌，鳞状细胞, 上皮-间质转化, 血管生成拟态
Objectives

To study the existence of epithelial-mesenchymal transition (EMT) and vasculogenic mimicry (VM) in human tongue squamous cell carcinoma (TSCC) and to elucidate the relationship between the EMT and VM.

Methods

Vimentin and E-cadherin were detected in 65 cases of TSCC and the adjacent normal tissue with immunohistochemistry. The presence of VM was detected with double stain of CD34 and PAS. The clinical significance of EMT and VM were then analyzed statistically.

Results

The positive rates of Vimentin in normal epithelial tissue and TSCC were 0% and 41.54%, respectively, with a significant difference between the two groups (Z=-5.815, P= 0.000) . Vimentin was closely correlated with tumor grade, clinical stages and lymphatic metastasis in TSCC. The positive rates of E-cadherin expression in normal epithelial and TSCC were 100% and 56.92%, respectively, with a significant difference between the two groups (Z=-5.951, P= 0.000) . The expression of E-cadherin protein was closely correlated with clinical stages and lymphatic metastasis in TSCC. The positive rates of VM in normal tissue and TSCC were 0% and 33.14%, respectively, with a significant difference between the two groups (Z=-3.946, P= 0.000) . The VM was closely correlated with clinical stages and lymphatic metastasis in TSCC. Bivariate analysis showed that E-cadherin was negatively correlated with Vimentin (r=-0.507, P= 0.000) . Vimentin was positively correlated with vasculogenic mimicry (r= 0.592, P= 0.000) , and E-cadherin was negatively correlated with vasculogenic mimicry (r=-0.518, P= 0.000) .

Conclusions

VM and EMT exist in TSCC and correlated with the lymphatic metastasis. VM was closely correlated with EMT, both of them had significant predictive value for the prognosis of NPC. The cancer with VM and EMT had stronger ability of metastasis and higher clinical stage. The EMT was closely correlated with VM, both of them have a important role in invasion and metastasis.

Key words: Tongue, Carcinoma, squamous cell, Epithelial-mesenchymal transition, Vasculogenic mimicry
表1 舌鳞状细胞癌Vimentin、E-cdherin和VM与临床病理特征的关系（例）
临床病理参数 例数 Vimentin表达 E?cadherin表达 VM情况
- + P值 - + P值 不存在 存在 P值
组织类型 ? ? ? 0.000 ? ? 0.000 ? ? 0.000
? 癌旁正常上皮 65 65 0 ? 0 65 ? 65 0 ?
? 鳞癌 65 38 27 ? 28 37 ? 51 14 ?
年龄（岁） ? ? ? 0.708 ? ? 0.356 ? ? 0.549
? < 40 13 7 6 ? 7 6 ? 11 2 ?
? ≥40 52 31 21 ? 21 31 ? 40 12 ?
性别 ? ? ? 0.594 ? ? 0.862 ? ? 0.178
? 男 41 25 16 ? 18 23 ? 30 11 ?
? 女 24 13 11 ? 10 14 ? 21 3 ?
临床分期 ? ? ? 0.020 ? ? 0.008 ? ? 0.011
? Ⅰ+Ⅱ期 42 29 13 ? 13 29 ? 37 5 ?
? Ⅲ+Ⅳ期 23 9 14 ? 15 8 ? 14 9 ?
组织学分级 ? ? ? 0.026 ? ? 0.579 ? ? 0.454
? 高分化 50 28 22 ? 20 30 ? 41 9 ?
? 中分化 12 10 2 ? 6 6 ? 8 4 ?
? 低分化 3 0 3 ? 2 1 ? 2 1 ?
淋巴结转移 ? ? ? 0.025 ? ? 0.008 ? ? 0.023
? 无 48 32 16 ? 16 32 ? 41 7 ?
? 有 17 6 11 ? 12 5 ? 10 7 ?
图1 发生上皮-间充质细胞转化的舌鳞状细胞癌出现Vimentin表达（EnvisionTM × 400）
图2 发生上皮-间充质细胞转化的舌鳞状细胞癌出现E-cadherin表达减弱或缺失（EnvisionTM × 400）
图3 舌鳞状细胞癌中存在血管生成拟态（箭头所示，CD34-PAS × 400）
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